新感溫自我硬化型磷酸鈣骨填補材之合成與特性

Synthesis and Characteristics of a New thermo-setting Calcium Phosphate Cement

鄧乃嘉(Nai-Chia Teng) ； 黃濬賢(Chun-Hsien Huang) ； 李勝揚(Sheng-Yang Lee) ； 楊正昌(Jen-Chang Yang)

《中華牙醫學雜誌（中文版）》 25卷3期 (2006/09) Pp. 157-168

https://doi.org/10.7106/CJD.200609.0157

摘要

針對自我固化(self-setting)型之人工合成磷酸鈣(calcium phosphate)骨填補材(bone graft)在骨缺損手術之臨床應用時，常因體液中之鎂離子延緩氫氧基磷灰石(hydroxyapatite, HA)之形成，致使骨填補材未及固化即遭體液沖刷而崩解，因此本研究目的在應用感溫型水膠開發具有快速固化之磷酸鈣骨填補材，以濕式化學沉澱法分別備製非結晶性磷酸鈣(amorphous calcium phosphate, ACP)與磷酸二鈣(dicalcium phosphate dihydrate, DCPD)粉體，藉由X-ray繞射分析儀、傅氏紅外光譜儀、場發射掃描式電子顯微鏡與能譜儀分析確認粉體的結晶形態、結構及鈣磷比值等物性，應用β-磷酸甘油(β-glycerol phosphate)及幾丁聚醣(chitosan)所組成的感溫型水膠，形成三維網狀(network)結構，讓ACP與DCPD粉體在凝膠環境保護下進行水合(hydration)固化反應。將磷酸鈣粉體與水膠溶液，依1g: 1ml的比例混合後注入直徑6mm高12mm之鐵氟龍圓柱模具中30秒後，將脫模的樣品置於37℃的漢克平衡鹽緩衝溶液中，結果顯示含β-磷酸甘油／幾丁聚醣水膠之磷酸鈣骨填補材具有抗沖刷(antiwash-out)特性。

關鍵字

磷酸鈣骨水泥 ； 非晶型磷酸鈣 ； 溫度敏感型水膠

並列摘要

Due to their excellent biological properties such as biocompatibility, bioresorbability, and osteoconductivity as well as their ease of use by injection, self-setting calcium phosphate cements (CPCs) are one of the most important artificial bone filling materials currently available. However, current products suffer from long setting times and thus are easily washed out. This washout phenomenon is the result of the slow formation of hydroxyapatite (HA), mainly caused by magnesium ions which exist in the body fluid and blood. To reach our goal of the preparation of an anti-washout CPC, a temperature-sensitive hydrogel, composed of amorphous calcium phosphate (ACP), dicalcium phosphate dihydrate (DCPD), β-glycerol-phosphate, and chitosan, was prepared with the mixture composition of 1 g:1 ml of solid: liquid. Our results showed that with the formation of a 3D network structure, the prepared samples were self-contained and resisted collapse and dissolution. With this improved anti-washout property, a better degree of hydration of the CPC was achieved.

並列關鍵字

calcium phosphate cement ； amorphous calcium phosphate ； temperature-sensitive hydrogel