- 期刊
Effect of Calcium Sulfate on Osteopontin Expression in U-2 Osteosarcoma Cells
並列摘要
Calcium sulfate has been used for bone regeneration in oral medicine. Osteopontin, a marker molecule of osteoblasts, plays multiple roles in bone formation. However, the effect of calcium sulfate on osteopontin expression in osteoblasts remains unclear. In this study, U-2 osteosarcoma (U-20S) osteoblast-like cells were cultured in medium containing 0, 0.5, 1, or 10 μM calcium sulfate hemihydrate for 6 days. The messenger (m) RNA and protein expressions of osteopontin were respectively analyzed by reverse-transcription polymerase chain reaction (RT-PCR) and Western blotting. In the in vitro culture for 1-2 days, there was no effect on the proliferation of cells, suggesting that calcium sulfate at up to 10μM is biocompatible. RT-PCR results showed that a lower concentration of calcium sulfate (＜ 1μM) upregulated the osteopontin mRNA level of U-2OS cells. However, the protein expression of osteopontin was downregulated as the concentration of calcium sulfate increased in cultured U-2OS osteoblastic cells. Dissolved •Ca(superscript 2+) and SO(superscript 2- subscript 4) from calcium sulfate hemihydrates may alter the structure of secreted osteopontin, which then was not recognizable by the antibody in the Western blot analysis. In summary, calcium sulfate can regulate the mRNA and protein expressions of osteopontin. Controlling the degradation of calcium sulfate implants is important for regulating bone formation. This study provides additional information to help dentists understand the effects of calcium sulfate on bone formation and use calcium sulfate more efficiently in treating bone defects.
被引用紀錄
延伸閱讀
- 張淵傑（2008）。The effect of calcium sulfate on the gene and protein expressions of osteopontin in U-2 osteosarcoma cells〔碩士論文，臺北醫學大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0007-2701200814224500
- 周佳璇（2019）。The Effects and Underlying Mechanisms of Dihydromyricetin on Human Osteosarcoma Cells Invasion and Metastasis〔博士論文，中山醫學大學〕。華藝線上圖書館。https://doi.org/10.6834/CSMU201900071
- 林任家（2020）。Study of apoptosis effect of licochalcone A in human osteosarcoma cells〔博士論文，中山醫學大學〕。華藝線上圖書館。https://doi.org/10.6834/csmu202000070
- Huang, W. H. (2010). Regulation of osteopontin expression by hypoxia in prostate cancer cells [master's thesis, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU.2010.00050
- Wagner, E. R., Luther, G., Zhu, G., Luo, Q., Shi, Q., Kim, S. H., Gao, J. L., Huang, E., Gao, Y., Yang, K., Wang, L., Teven, C., Luo, X., Liu, X., Li, M., Hu, N., Su, Y., Bi, Y., He, B. C., Tang, N., Luo, J., Chen, L., Zuo, G., Rames, R., Haydon, R. C., Luu, H. H., & He, T. C. (2011). Defective Osteogenic Differentiation in the Development of Osteosarcoma. Sarcoma, 2011(), 116-127. https://doi.org/10.1155/2011/325238