In Vitro Production of Cytokines and Allergen-Specific IgE in Bronchial Asthmatic Children with Different Disease Activity

淋巴介質及過敏原特異性IgE抗體在不同病程支氣管性氣喘病童的表現

江伯倫(Bor-Luen Chiang) ； 蔡銘哲(Ming-Jer Tsai) ； 周正成(Chen-Cheng Chou) ； 謝貴雄(Kue-Hsiung Hsieh)

《Acta Paediatrica Sinica》 39卷3期 (1998/06) Pp. 173-179

https://doi.org/10.7097/APS.199806.0173

摘要

氣喘病在近年有逐年增加的趨勢，而在過敏疾病的發病機轉中又以第二型T輔助細胞及相關的淋巴介質扮演了一個重要的角色。為了更進一步研究在不同病程的氣喘病患童過敏原特异性抗體和灑巴介質的變化，我們分別收集了剛診斷（21位）、急性發作（17位）、接受減敏感治療反應良好（20位）及不佳（12位）和正常孩童（20位）等五組，測定其周邊血液單核球所製造的淋巴介質和過敏原抗體的濃度。其結果包括 1)IgE抗體的濃度的地減敏治療反應不良的患童最高，相對的，IgE4抗體在反應良好的患童最高；2)減敏治療反應良好的氣喘病童其周邊血液單核球製造的IFN-γ較高，相對的，IL-4的濃度則在初診斷的病童最高；3)IL-13的濃度則在減敏治療反應不良的氣喘病童最高；4)接受減敏治療的氣喘病童，不論結果良好或是不佳，其IL-10的濃度則來得較低。我　們一直相信只有對過敏疾病的發病機轉及減敏治療的機制有更進一步的了解，將有助於我人產對未來的治療研究的更進一步的幫忙。

關鍵字

氣喘 ； 淋巴介質 ； 減敏療法 ； 免疫球蛋白E

並列摘要

The aim of this study was to further investigate the cytokine profile and the level of allergen-specific immunoglobulin E (IgE) and IgG4 in asthmatic children of different disease activity. Peripheral blood mononuclear cells were isolated from seventy asthmatic children who had been newly diagnosed (N=21), had good (N=20) or poor response (N=12) to immunotherapy or during acute attack (N=17) and from twenty normal children. Cytokines produced by both PHA and mite allergen stimulated peripheral blood mononuclear cells (PBMCs) were assayed. Resulting data suggested: 1) the level of IgE anti-house dust mite (HDM) antibody was higher in asthmatic children with poor response to immunotherapy; in contrast, among the five groups the level of IgG4 anti-HDM antibody was the highest in asthmatic children with good response to immunotherapy among the five groups; 2) the level of interferon-γ (IFN-γ) produced by mite-stimulated PBMCs was the highest in asthmatic chidren with good response to immunotherapy among the five groups; in contrast, the level of interleukin-4 (IL-4) was the highest in the group of newly diagnosed asthmatic children; 3) the level of IL-13 was higher in asthmatic children with poor response to immunotherapy compared to those of the other groups; 4) a higher level of IL-1O and lower level of IL-12 were noted in groups of both good and poor response to immunotherapy compared to those of other groups. Although more studies are needed, the change of cytokines in asthmatic children with different disease activity or response to immunotherapy might shed light on further understanding of the regulatory mechanisms of atopic diseases.

並列關鍵字

asthma ； cytokines ； immunotherapy ； immunoglobulin E