利用臺灣蜆(Corbicula fluminea)來監測水中類雌性素物質－雙酚A(bisphenol A)之初步探討

Application of Corbicula fluminea Monitoring Aquatic xenoestrogen-bisphenol a

謝宗志(Zong-Jhih Sie) ； 萬鑫偉(Xin-Wei Wan) ； 黃大駿(Da-Ji Huang)

《貝類學報》 33期 (2009/12) Pp. 55-66

https://doi.org/10.29998/BM.200912.0005

摘要

臺灣蜆(Corbicla fluminea)是屬於高耐污力、雌雄同體的物種，非常適合作為毒性研究的實驗物種。本研究為了瞭解臺灣蜆是否適合應用監測水中類雌性素(xenestrogen)之指標生物，利用水中常見之類雌性素一雙醃A(bisphenol A, BPA)作為外因性雌性素之試驗用藥，除此之外亦利用17β-estradiol作為比較。實驗中應用不同濃度的BPA曝露臺灣蜆後，於1、3及7天，測定臺灣蜆體內vitellogenin(VTG)濃度來探討BPA對臺灣蜆是否存在類雌性素的功能。試驗同時為了有效探討毒性危害，利用monooxygenase(MO)、glutathione-S-transferase(GST)、aspartate aminotransferase（GOT或AST）及alanine aminotransferase（GPT或ALT）來觀察BPA對臺灣蜆的毒性影響。結果顯示BPA對於臺灣蜆雖有毒性反應，但沒有明顯的毒性危害，同時BPA會誘發臺灣蜆體內VTG生成。實驗初步證實臺灣蜆應可應用於監測水環境中類雌性素物質。

關鍵字

臺灣蜆Corbicula fluminea ； 類雌性素 ； 雙酚A ； 卵黃前質素

並列摘要

The purpose of this study was to investigate the probably of monitor xenoestrogen in Corbicula fluminea. Individuals of C. fluminea, a common inhabitant of freshwater systems in Taiwan, was exposed to different levels of xenoestrogen-bisphenol A (BPA; 1, 10 and 100 μg L^(-1)) and 100 μg L^(-1) 17β-estradiol, positive control. The detoxication signals which active of monooxygenase (MO) and glutathione-S-transferase (GST), heaptopancreas damage signals which active of aspartate aminotransferase (GOT or AST) and alanine aminotransferase (GPT or ALT), and vitellogenin (VTG), xenoestrogen bioindicator, measured after 1、3 and 7 exposure. According to our findings, induction of a VTG is observed in BPA-treated clam, and not serious detoxication signals and heaptopancreas damage signals in BPA- and 17β-estradiol -treated clam. Our result demonstrated that C. fluminea have high potential for monitoring xenoestrogen especially-bisphenol A.

並列關鍵字

Corbicula fluminea ； xenoestrogen ； bisphenol A ； vitellogenin