比較大鼠氣管灌注蘇力菌及黑殭菌袍子之清除及影響

Clearance and Effects of Intratracheal Instillation to Spores of Bacillus thuringiensis or Metarhizium anisopliae in Rats

蔡三福(San-Fu Tsai) ； 廖俊旺(Jiunn-Wang Liao) ； 王順成(Shun-Cheng Wang)

《中華民國獸醫學會雜誌》 23卷6期 (1997/12) Pp. 515-522

https://doi.org/10.7009/JTCSVS.199712.0515

摘要

以純化的微生物製劑之蘇力菌（Bacillus thuringiensis）或黑殭菌（Metarhizium anisopliae）1X10^8個活孢子對大鼠進行單一氣管內灌注，評估其在哺乳類動物肺臟內之清除及其影響情形。結果灌注孢子之處理大鼠增重較對照組下降，白血球及紅血球總數、紅血球濃度、血容比均增加。蘇力菌或黑殭菌之孢子肺臟回收培養試驗中，其總菌落數少於接種之孢子數，蘇力菌處理組其孢子分佈於肺、肝、脾、腎及腸系膜淋巴組織，且孢子在組織中存活之時間超過21天，而黑殭菌孢子只分佈於接種部位之肺臟組織，孢子在組織中存活時間不足14天。組織病理可見在白鼠氣管內灌注不同的孢子懸浮液後，均造成支氣管周圍及肺泡細胞大量的炎症細胞浸潤，黑殭菌孢子所造成的病變比蘇力菌孢子顯著，並形成肥大的泡沫狀細胞，而蘇力菌孢子主要形成肉芽腫組織病變。對大鼠進行單一氣管內灌注的試驗過程中，雖未發現兩種孢子於組織中產生感染增殖的跡象，唯當暴露於高劑量下，仍需再進一步詳細評估其對人類健康潛在的風險性。

關鍵字

蘇力菌 ； 黑殭菌 ； 鼠 ； 氣管灌注

並列摘要

This work investigated the clearance and effects of spores of B. thuringiensis (Bt) and M. anisopliae (Ma) in rats after single intratracheal administrations of 1 X 10^8 spores in phosphate-buffered saline. In rats injected with spores, the body weight gain was decreased and leucocyte and erythrocyte counts, hemoglobin concentration, and hematocrit were increased, the recovery culture examination and histological studies revealed that the microbial of total number of colony forming unit recovered less than the initiated inoculation. The distribution of Bt spores were found not only in the lung but also in liver, spleen, kidney and mesenteric Iymph nodes. However, but Ma spores presented only in the lung. The spore of Bt survived in tissue up to 21 day, and Ma 14 days only after administration of the dose. The rats receiving spores of Ma intranasally developed severe inflammatory changes within both bronchioles and alveoli, subsequently forming foamy cells in the lungs. The rats receiving spores of 8t also developed inflammatory changes in the lung, but the foamy cells were less than those receiving spores of Ma, and formed focal or multifocal granulomatous pneumonitis. No spore-germination was found in the rat receiving 1 X 10^8 spores of Bt or Ma. Nevertheless, our findings confirm that exposure to highly dosage spores of Bt or Ma can be a health risk.

並列關鍵字

Bacillus thuringiensis ； Metarhizium anisopliae ； Rats ； Intratracheal ； Instillation