Comparison of Safety and Efficacy of Erysipelas Inactivated and Attenuated Vaccines in Mice and Pigs

試製之豬丹毒不活化菌苗與弱毒活菌苗，以小白鼠及豬隻作安全及免疫效力試驗之結果，得知兩者對供試動物均無不良之接種反應。小白鼠即使注射10倍豬免疫劑量之弱毒活菌苗亦無任何不良反應，而接種1.4 X 10^4 CFU/Mouse/SC即可耐過100 MLD之攻擊。另一方面不活化菌苗，其安全性雖甚佳，但免疫效力要達耐過100 MLD之攻擊，則需3 X 10^10 CFU/Mouse/SC以上。4頭豬隻免疫接種弱毒活菌苗2週後血清，對豬丹毒強毒株（1a）發育凝集抗體價（G A titer）達1：32，攻擊後2週其抗體價亦無明顯改變。但6頭豬隻接種不活化菌苗2週後其血清中發育凝集抗體價均為1：8，攻擊後2週則達1：128-1：512不等。此等高抗體價顯然是攻擊耐過之免疫反應。由試驗結果顯示，弱毒活菌苗效果優於不活化菌苗。

關鍵字

豬丹毒；不活化菌苗；弱毒活菌苗；免疫效力

並列摘要

Mice and pigs were used to test the safety and efficacy of mono-and poly-valent inactivated vaccines and mono-valent attenuated vaccine of erysipelas. The results showed no side reactions on either experimental animals, even when 10 times doses for pigs were used in mice. When the mice were immunized, subcutaneous, with 1.4 X 10^4 CFU of attenuated vaccine they resisted to the challenge of 100 MLD of a virulent Erysipelothrix rhusiopathiae (Er). Although the inactivated vaccine is very sale, a vaccination dose of 3 X 10^10 CFU was required for the protection from the 100 MLD challenge. On the other hand, the growth agglutination (GA) titers of four pigs were 1:32 when the sera were collected at two weeks after vaccination using attenuated vaccine. In addition, no distinct changes in the GA titers were found at two weeks after challenge (4 weeks after vaccination). However, the GA titers were 1:8 for other 6 pigs when the sera were tested at 2 weeks after vaccination with inactivated vaccine. This titers increased to 1:128 to 1:512 when the sera were tested at two weeks after challenge. These increased titers were apparently due to the exposure of pigs to challenge Er. The results indicated that the efficacy of the attenuated vaccine is better than that of the inactivated bacterin.

並列關鍵字

Erysipelas ； Inactivated bacterin ； Attenuated vaccine ； Immunity potency

國際替代計量

Comparison of Safety and Efficacy of Erysipelas Inactivated and Attenuated Vaccines in Mice and Pigs

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