基因工程產製豬白血球中介質Ⅱ對豬隻接種豬生殖與呼吸綜合症死毒免疫效力的影響

The Effect of Recombinant Porcine Interleukin-2 on the Immune Responses to Inactivated Porcine Reproductive and Respiratory Syndrome Virus in Swine

蕭世烜(Shih-Hsuan Hsiao) ； 鄭謙仁(Chian-Ren Jeng) ； 張志成(Chih-Cheng Chang) ； 闕玲玲(Ling-Ling Chueh) ； 鄭益謙(Ivan-Chen Cheng) ； 楊平政(Ping-Cheng Yang) ； 龐飛(Victor Fei Pang)

《中華民國獸醫學會雜誌》 25卷1期 (1999/03) Pp. 23-33

https://doi.org/10.7009/JTCSVS.199903.0023

摘要

The objective of the study was to evaluate the effect of recombinant porcine interleukin-2 (rpIL-2) on the immune responses of porcine reproductive and respiratory syndrome virus (PRRSV) infected pigs immunized with inactivated PRRSV. Sixteen age-matched anti-PRRSV antibody positive pigs were randomly assigned to 4 groups. Two of the 4 groups received 2 doses of heat-inactivated PRRSV (106 TCID50) mixed with aluminum hydroxide within an interval of 3 weeks; 105U of rpIL-2 was also added to the heat-inactivated PRRSV preparation of 1 of the 2 groups. The 2 immunized groups and 1 unimmunized group were challenged intranasally with 104 TCID50 PRRSV 3 weeks after the 2nd immunization. The remaining group, neither immunized nor challenged, served as the environment control group. The immune responses, including virus neutralizing antibody titer, peripheral blood lymphocyte blastogenesis and peripheral blood mononuclear cell cytotoxicity against virus infected cells as well as phagocytosis of yeast, superoxide anion release, and tumor necrosis factor production by alveolar macrophages, as well as clinical observations, including symptoms, daily weight gain, mortality, and virus shedding, were examined and recorded. Upon comparsion, the group receiving heat-inactivated PRRSV and rpIL-2 showed significant (P＜0.05) elevations on the non-specific lymphocyte blastogenesis on day 3 after the 1st and 2nd immunizations, and virus-specific lymphocyte blastogenesis on day 14 after the 2nd immunization without observable clinical alterations; however, there were no significant changes in virus neutralizing antibody titer, peripheral blood mononuclear cell cytotoxicity or phagocytosis of yeast, superoxide anion release, and tumor necrosis factor production by alveolar macrophages.

關鍵字

豬生殖與呼吸綜合症 ； 基因工程產製豬白血球中介質Ⅱ ； 免疫力 ； 豬

並列摘要

The objective of the study was to evaluate the effect of recombinant porcine interleukin-2 (rpIL-2) on the immune responses of porcine reproductive and respiratory syndrome virus (PRRSV) infected pigs immunized with inactivated PRRSV. Sixteen age-matched anti-PRRSV antibody positive pigs were randomly assigned to 4 groups. Two of the 4 groups received 2 doses of heat-inactivated PRRSV (106 TCID50) mixed with aluminum hydroxide within an interval of 3 weeks; 105U of rpIL-2 was also added to the heat-inactivated PRRSV preparation of 1 of the 2 groups. The 2 immunized groups and 1 unimmunized group were challenged intranasally with 104 TCID50 PRRSV 3 weeks after the 2nd immunization. The remaining group, neither immunized nor challenged, served as the environment control group. The immune responses, including virus neutralizing antibody titer, peripheral blood lymphocyte blastogenesis and peripheral blood mononuclear cell cytotoxicity against virus infected cells as well as phagocytosis of yeast, superoxide anion release, and tumor necrosis factor production by alveolar macrophages, as well as clinical observations, including symptoms, daily weight gain, mortality, and virus shedding, were examined and recorded. Upon comparsion, the group receiving heat-inactivated PRRSV and rpIL-2 showed significant (P＜0.05) elevations on the non-specific lymphocyte blastogenesis on day 3 after the 1st and 2nd immunizations, and virus-specific lymphocyte blastogenesis on day 14 after the 2nd immunization without observable clinical alterations; however, there were no significant changes in virus neutralizing antibody titer, peripheral blood mononuclear cell cytotoxicity or phagocytosis of yeast, superoxide anion release, and tumor necrosis factor production by alveolar macrophages.

並列關鍵字

Porcine reproductive and respiratory syndrome ； Recombinant porcine interleukin-2 ； Vaccination