Capillary PCR for Detecting and Differentiating Feline Panleukopenia Virus and Canine Parvovirus in Fecal Specimens

以毛細管聚合酶鏈反應偵測並區分糞便檢體中犬小病毒及貓汎白血球缺乏症病毒

潘銘正(Ming-Jeng Pan) ； 張溫嵐(Wen-Lan Chang) ； 蘇蕙莉(Whey-Li Su)

《中華民國獸醫學會雜誌》 26卷1期 (2000/03) Pp. 14-23

https://doi.org/10.7009/JTCSVS.200003.0014

摘要

本試驗以毛細管聚合酶鏈反應偵測並區分糞便檢體中犬小病毒及貓汎白血球缺乏症病毒。偵測用增幅引子序列來自兩種病毒VP1/VP2保留區，區分用增幅引子序列則來自外殼蛋白中決定感染宿主範圍的部份。其敏感度比病毒分離培養及血球凝集試驗高100-10,000倍。為建立適當臨床糞便檢體檢驗步驟，本試驗收集50個疑似感染犬小病毒病例之糞便檢體，分別以不同方法抽取病毒核酸，進行毛細管聚合酶鏈反應，並同時進行病毒分離培養及血球凝集試驗。結果證明毛細管聚合酶鏈反應具高敏感度及高特異性，可有效偵測並區分糞便檢體中犬小病毒及貓汎白血球缺乏症病毒，因此亦可監測犬貓之間的相互傳染。

關鍵字

犬小病毒 ； 貓汎白血球缺乏症病毒 ； 毛細管聚合酶鏈反應

並列摘要

We have developed a capillary polymerase chain reaction (PCR) method to detect both canine parvovirus (CPV) and feline panleukopenia virus (FPLV) as well as to differentiate these two viruses in fecal specimens. The sequences of the PCR primer pair for detection were selected from the conserved region in the VP1/VP2 gene of both viruses. The PCR for differentiation is based on a difference between nucleotide sequences of the capsid protein gene of CPV and FPLV that determines the canine host range of CPV. The result of sensitivity tests demonstrates our PCR assay was 100- to 10,000-fold higher than that of the culture method and the hemagglutination (HA) test. To establish a proper procedure for preparing clinical samples, we compared the results of the PCR assay performed with fifty fecal samples taken from dogs suspected of CPV infection that underwent differnt DNA extraction methods. In addition, these samples were also examined by other standard assays for CPV infection, and the results were compared with this PCR assay. We conclude that this capillary PCR system is a highly specific and sensitive diagnostic method for CPV and FPV infection, which is also potential to monitor the interspecies transmission of these ever changing viruses between dog and cats efficiently.

並列關鍵字

Canine parvovirus ； Feline panleukopenia virus ； Capillary polymerase chain reaction