Using a Radioactive and Non-Radioactive Exchangeable Labeling System for Laboratory Diagnosis

本文中建立了一種安全簡易之探針標定方法，應用於臨床病原菌及致癌基因等之鑑定。於南方及北方墨點之診斷中，同一張轉印膜只需經簡易之水洗處理即可進行放射性或非放射性探針互換之標定“由於非放射性探針標定之安全性高，操作容易，曝光時間短，可做為初步之診斷鑑定，而當非放射性探針之標定能力較弱時，一些微量之訊息無法偵測到，此時在同一張轉印膜上再換以放射性探針偵測之。實驗顯示，於最佳之探針雜交條件下，放射性或非放射性探針標定能力相當，於臨床診斷時可先以非放射性之探針鑑定，若無法偵測到目標物時，再以放射性探針做進一步之鑑定，同時一張轉印膜於水洗後，可用不同之探針標定並連續使用之。

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並列摘要

In this communication a safe and simple molecular diagnosis strategy was described, which can be applied for the identification of pathogens or oncogenes in clinical studies. In Southern or Northern analysis, we demonstrated the feasibility to exchange labeling probes from non-radioactive to radioactive or vice versa in a single membrane simply by stripping. We chose non-radioactive labeling because it is safe and easy to operate, but when the sensitivity is insufficient, it can be replaced by radioactive labeling. Our data show that radioactive or non-radioactive labeling is exchangeable in a single membrane and the sensitivity was comparable by either method under optimal conditions. The clinical implication is that for general hybridization starts with non-radioactive labeling, radioactive labeling is not needed unless the signals cannot be detected by non-radioactive labeling. Moreover, one membrane can be labeled repeatedly by different probes.

並列關鍵字

Hybridization ； Non-radioactive labeling ； Radioactive labeling

國際替代計量

Using a Radioactive and Non-Radioactive Exchangeable Labeling System for Laboratory Diagnosis

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