Suppression of Isoproterenol-Induced Apoptosis in H9c2 Cardiomyoblast Cells by Daidzein through Activation of Akt

Wei-Syun Hu ； Yueh-Min Lin ； Wei-Wen Kuo ； Lung-Fa Pan ； Yu-Lan Yeh ； Yi-Hui Li ； Chia-Hua Kuo ； Ray-Jade Chen ； V. Vijaya Padma ； Tung-Sheng Chen ； Chih-Yang Huang

《The Chinese Journal of Physiology》 59卷6期 (2016/12) Pp. 323-330

https://doi.org/10.4077/CJP.2016.BAE393

摘要

Increased serum norepinephrine level is one of pathological processes relating to heart disease (HD). Estrogens are considered as potential therapeutics for the treatment of HD; however, estrogen supplementation shows some side-effects, such as increasing the risk of developing breast, endometrial and ovarian cancers. This study investigated the cardio-protective effects of daidzein (Dai), a selective estrogen receptor modulator (SERM) from soy bean extract, in H9c2 cardiomyoblast cells treated with isoproterenol (ISO), a norepinephrine analog. In this in vitro model, H9c2 cells treated with Dai at different concentrations showed no statistical difference in cell viability. TdT-mediated digoxigenin-dUTP nick-end labeling (TUNEL) data and western blotting results indicated that Dai treated-H9c2 cells recovered from the damage induced by ISO. The recovery effects of Dai on ISO-induced damage were blocked by inhibition of Akt activation through adding Akt inhibitor. On the other hand, the fold changes of phosphorylated Akt (p-Akt)/Akt normalized with the control for con, 0.25, 0.5, 1, 3 and 24 h of treatment were 1, 2, 5, 13, 11 and 10, respectively. In conclusion, Dai ameliorates apoptosis of cardiomyoblasts induced by ISO through Akt signaling pathway.

關鍵字

apoptosis ； daidzein ； estrogen ； heart disease ； isoproterenol

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