Various Microscopic Methods for Investigating the Venuloid Idioblasts of Pteris grevilleana Wall

Tzu-Tong Kao ； Shiang-Jiuun Chen ； Wen-Liang Chiou ； Yi-Chun Chuang ； Ling-Long Kuo-Huang

《TAIWANIA》 53卷4期 (2008/12) Pp. 394-399

https://doi.org/10.6165/tai.2008.53(4).394

並列摘要

Venuloid idioblasts are vein-like structures. In Pteris, they are long epidermal cells with very thick cell walls. In this study, venuloid idioblasts of Pteris grevilleana were investigated with various light microscopic (LM) and scanning electron microscopic (SEM) techniques and the main purposes of these microscopic techniques are summarized and discussed. To investigate the morphology and distribution of venuloid idioblasts, partial polarization LM technique and cryo-tabletop-SEM technique were used. The idioblasts had lobed margins, acute or round ends, and could be found on both upper and lower epidermis of fertile fronds and sterile fronds. They are distributed on veins, interveinal regions, and leaf margins, but not on costules, costae, and false indusia. By using histochemical staining and SEM with energy dispersive X-ray spectrometer (EDS), it was confirmed that the idioblasts contained silicon. In addition, the venuloid-idioblast-like silica bodies were extracted by wet oxidation method. These indicate that the venuloid indioblast in P. grevilleana is a kind of spicular cell (long epidermal cells containing silica bodies, which are found in Adiantoids and Vittarioids).

並列關鍵字

EDS ； false vein ； partial polarization ； Pteridaceae ； Pteris grevilleana ； silica body ； spicular cell ； tabletop SEM ； venuloid idioblast ； wet oxidation