- 期刊
Rhizobia symbiosis of seven leguminous species growing along Xindian riverbank of Northern Taiwan
摘要
Legume-rhizobia symbioses of seven leguminous species growing along Xindian riverbank of Northern Taiwan were investigated in this study. These legumes form either determinate or indeterminate types of root nodules. The determinate nodules of Alysicarpus vaginalis, Desmodium. triflorum, D. heterophyllum, Sesbania cannabina and the indeterminate nodules of Mimosa pudica harbored bacteroids of morphological uniformity (length of 1-3 μm), while the indeterminate nodules of Crotalaria zanzibarica and Trifolium repens contained bacteroids of highly pleomorphism (size varying from 1 to 5 μm). The enclosed bacteria were isolated from respective nodules, and twenty slow-growing and nine fast-growing rhizobial isolates were recovered. The slow-growing isolates were classified to the genus Bradyrhizobium based on the 16S rRNA sequences, whereas the fast-growing rhizobia comprise four genera, Neorhizobium, Rhizobium, Cupriavidus and Paraburkholderia. Results of stable isotope analyses revealed that the seven leguminous species had similar and consistently negative δ^(15)N values in leaves (mean of -1.2 per mille), whereas the values were positive (varying from 3.7 to 7.3 per mille) in the nodules. These values were significantly higher in the indeterminate nodules than those in the determinate ones. In addition, variations in the values of leaf δ^(13)C (varying from -29 to -34 per mille) among the seven legumes were measured, indicating their photosynthetic water use efficiencies were different. This is the first field survey to report the rhizobial diversity and the nutrient relationships of sympatric legume in Taiwan.
參考文獻
延伸閱讀
- Wu, J. T. (1999). Occurrence of Four Freshwater Rhodophytes in Taiwan. TAIWANIA, 44(1), 145-153. https://doi.org/10.6165/tai.1999.44(1).145
- Wu, M. L. (1989). Zygospore Formation of Rhizopus Stolonifer from Taiwan. Transactions of the Mycological Society of Republic of China, 4(1), 59-64. https://doi.org/10.7099/TMSRC.198903.0059
- 顏江河、鍾旭和（1990）。Observations on the Endomycorrhizae of Taiwania cryptomeriodes Infected with Scutellospora nigra。林業試驗所研究報告季刊，5(1)，53-62。https://doi.org/10.7075/BTFRI.199003.0053
- 胡若梅、童武夫（1989）。Infection of Tumor-Induced Bacteria on Some Species of Plants in Taiwan。師大生物學報，24()，125-135。https://doi.org/10.29812/BBNTNU.198912.0008
- Chen, S. F. (2007). Anisolpidium saprobium and Rhizidiomyces Hirsutus, New Records of Hyphochytriomycetes (Hyphochytriales) in Taiwan. Fungal Science, 22(3&4), 79-83. https://doi.org/10.7099/FS.200712.0079