Efficacy of Using Schizophyllan on Innate Immune Responses and Disease Resistant of ＂Epinephelus coioides＂

裂褶菌多醣對多醣對點帶石斑魚非特異免疫反應與抗病能力的影響

張景盛(Ching-Sheng Chang) ； 冉繁華(Fan-Hua Nan) ； 陳秀男(Shiu-Nan Chen)

《臺灣水產學會刊》 40卷4期 (2013/12) Pp. 219-229

https://doi.org/10.29822/JFST.201312_40(4).0001

摘要

本研究的在探討裂褶菌多醣對於點帶石斑非特異免疫與抗病能力的影響，裂褶菌多醣是一種由裂褶菌醱酵所萃取的葡聚多醣體，研究的結果顯示，在點帶石斑魚餵食每公斤飼料內添加1.0與2.0公克的裂褶菌多醣7天後對於提高溶菌酶酵素活性，吞噬細胞吞噬活性，吞噬指標與呼吸爆均有顯著的影響，而在餵食每公斤飼料內添加1.0與2.0公克的裂褶菌多醣14天後以溶藻弧菌進行攻擊會有最佳的保護能力。本研究的結果顯示裂摺菌多醣對點帶石斑魚具有免疫調節的能力，即使提高劑量，也不會引發過度的免疫反應，是一種可以口服餵食的免疫調節物質。

關鍵字

菇蕈葡聚多醣 ； 裂褶菌多醣 ； 點帶石斑 ； 疾病控制能力 ； 非特異免疫反應

並列摘要

This study attempts to describe the effects of innate immune response and disease resistant of schizophyllan in orange-sported grouper, ＂Epinephelus coioides＂. The schizophyllan is a beta-glucan, and is an extraction from Schizophyllum commune fermentation. Study performed on the innate immune response showed that oral ingestion of schizophyllan at 1.0 g and 2.0 g per kilogram diets of feed levels after 7 days may significantly enhance the lysozyme activity, phagocytic activity, phagocytic index and respiration burst of the experimental groupers. Observation on the experimental challenge of pathogen showed that uses of 1.0 g and 2.0 g schizophyllan per kilogram diets in feed 14 days could significantly enhance the protection of grouper against ＂Vibrio alginolyticus＂. In this study, schizophyllan have been orally administered in grouper showing immunomodulation. Schizophyllan is an immunomodulatory of grouper, even if fed over-dosage, does not lead to over-regulation of the innate immune response.

並列關鍵字

Mushroom beta-glucan ； Schizophyllan ； Orange-spotted grouper ； Disease Resistant ； Innate immune response