臺灣一葉蘭之組織培養

Tissue Culture of Pleione formosana Hayata

張耀乾(Yao-Chien Chang) ； 李哖(Nean Lee)

《中國園藝》 38卷2期 (1992/06) Pp. 80-90

https://doi.org/10.6964/JCSHS.199206.0080

摘要

臺灣一葉蘭行組織培養，以秋末落葉採收之成熟碩大球莖，經l至2個月5℃冷藏後，取其花芽內莖頂爲培植體最易成功。1/3MS鹽類促進原球體的形成與增殖，在Knudson's C培養基下易有不定根之發生、原球體生成數少。NAA 1ppm或低濃度2，4-D有助於原球體之增生，當球莖冷藏後取花芽內之莖頂繁殖時，值物生長調節劑並非絕對必需。椰子水並無顯著效果，固體培養方式似優於液體培養。臺灣一葉蘭經組織培養可正常增殖、結球，其繁殖速率比無菌播種之實生繁殖爲快。

關鍵字

臺灣一葉蘭 ； 組織培養 ； 培植體 ； 植物生長調節劑 ； 基本鹽類 ； 低溫冷藏

並列摘要

The most suitable tissue of Pleione formosana Hayata for tissue culture was the shoot tip from flower bud of large and matured corm, which was harvested in winter and then stored at 5℃ for one to two months. Somatic protocorm formation and proliferation from shoot tip were promted by 1/3 MS basal salts. However, adventitious roots induced easily and few somatic protocorms were formed from shoot tip cultured on Knudson's C medium. After cold storage, the shoot tips had little response to plant growth regulators for somatic protocorm proliferation. The coconut milk did not make a significantly positive effect. The proliferationof shoot tip seemed to perform better in solid media than in liguid ones. The growth of plantlets and bulbing through tissue culture were faster than seedlings germinated from seeds.

並列關鍵字

Pleione formosana ； tissue culture ； explant ； plant qrowth regulators ； basal salts ； cold storage