光度對黛粉葉組織培養苗出瓶後生長與光合作用之影響

Effect of Photosynthetic Photon Flux on Growth and Photosynthesis of Micropropagated Dieffenbachia Plantlets

褚昱均(Yu-Chun Chu) ； 葉德銘(Der-Ming Yeh)

《中華農學會報》 7卷4期 (2006/08) Pp. 404-413

https://doi.org/10.6730/JAAC.200608_7(4).0006

摘要

將黛粉葉(Dieffenbachia 'Tropic Marianne')組織培養苗出瓶置於25±1℃之育苗室，每日分別以16小時之80、130或200μmol m^(-2)s^(-1)光度處理60天，再經過溫室栽培120天後調查後期之生長狀況。結果顯示以80μmol m^(-2)s^(-1)光度處理者枝條乾重較重。培植體在瓶內形成之葉片，在出瓶初期Fv/Fm值皆為下降趨勢，處理光度越高測值越低，在200μmol m^(-2)s^(-1)處理下Fv/Fm值降至0.55，之後回復較慢。培植體在瓶內形成葉片之蒸散速率和氣孔導度，在出瓶初期時皆有由較高值急速下降；但之後氣孔導度測值呈現回升趨勢。瓶內已形成葉片的淨光合作用速率，在剛出瓶時約為2.5μmol CO2 m^(-2)s^(-1)，在出瓶後1天下降，之後呈現回升趨勢。

關鍵字

黛粉葉 ； 光度 ； 蒸散作用 ； 氣孔導度

並列摘要

Dieffenbachia maculata Schott. 'Tropic Marianne' micropropagated plantlets were transferred to soil less mix and placed in a growth room at 25±1°C, under cool-white fluorescent light, providing 16 hours per day of photosynthetic photon flux (PPF) of 80, 130 or 200 μmol m^(-2) s^(-1). After being treated for 60 days in the growth room, the plantlets were then transferred to a shaded greenhouse for 120 days and their growth was measured Plantlets treated below 80 μmol m^(-2)s^(-1) had higher shoot dry weight than those under 130 or 200 μmol m^(-2) s^(-1). All the Fv/Fm values in the in vitro-formed leaf decreased in the early period after transfer, and then increased thereafter. High PPF at 200 μmol m^(-2) s^(-1) greatly reduced the Fv/Fm value to 0.55, clearly exhibiting photo inhibition and a slow increase in the Fv/Fm value afterwards. Stomatal conductance and transpiration rate values in the in vitro-formed leaf were declca3ed dramatically during the first few days after transfer, while stomatal conductance increased gradually thereafter. Photosyntrietic rate of the in vitro-formed leaf was measured as 2.5 μmol CO m^(-2) s^(-1) at transfer, decreased after transferring for one day, and then increased gradually.

並列關鍵字

Dieffenbachia ； photosynthetic photon flux ； transpiration ； stomatal conductance