Urinary Isoflavonoid Excretion as a Biomarker of Dietary Soy Intake during Two Randomized Soy Trials

为了评估尿液异黄酮的分泌作为膳食异黄酮摄入的生物标志物，我们在256位绝经前妇女中进行了两项随机大豆试验（13-24个月），共收集了1,385份重复尿样。参与者分别在不同时期摄入高大豆膳食（2份／天）和低大豆膳食（3份／周），完成7份随机的24小时膳食回顾，并提供重复的尿样用于异黄酮排泄量的分析（液相色谱法）。我们计算了Spearman相关系数并用logistic回归估计曲线下面积。基线、低和高大豆膳食期平均每日总膳食异黄酮的摄入量分别为2.3、0.2和60.4 mg糖苷配基当量，相应的尿中异黄酮的分泌分别为0.4、1.0和32.4 nmol/mg 肌酐。在组间，尿异黄酮分泌和膳食异黄酮摄入量显著相关（r_s = 0.51, AUC = 0.85, p ＜ 0.0001），但在组内无显著性（r_s = 0.05-0.06, AUC = 0.565-0.573）。尿异黄酮分泌是区分人群大豆膳食高低的一个很好的生物标志物。但随着大豆摄入量范围的缩小，尿异黄酮分泌与膳食异黄酮摄入的关联减弱。

關鍵字

大豆食品；大豆异黄酮；生物标志物；膳食干预；流行病学

並列摘要

We evaluated urinary isoflavonoid excretion as a biomarker of dietary isoflavone intake during two randomized soy trials (13-24 months) among 256 premenopausal women with a total of 1,385 repeated urine samples. Participants consumed a high-soy diet (2 servings/day) and a low-soy diet (＜ 3 servings/week), completed 7 unannounced 24-hour dietary recalls, and donated repeated urine samples, which were analyzed for isoflavonoid excretion by liquid chromatography methods. We computed Spearman correlation coefficients and applied logistic regression to estimate the area under the curve. Median overall daily dietary isoflavone intakes at baseline, during low- and high-soy diet were 2.3, 0.2, and 60.4 mg aglycone equivalents, respectively. The corresponding urinary isoflavonoid excretion values were 0.4, 1.0, and 32.4 nmol/mg creatinine. Across diets, urinary isoflavonoid excretion was significantly associated with dietary isoflavone intake (r_s = 0.51, AUC = 0.85; p ＜ 0.0001) but not within diet periods (r_s = 0.05-0.06, AUC = 0.565-0.573). Urinary isoflavonoid excretion is an excellent biomarker to discriminate between low- and high-soy diets across populations, but the association with dietary isoflavone intake is weak when the range of soy intake is small.

並列關鍵字

soy foods ； isoflavone ； biomarker ； dietary intervention ； epidemiology

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國際替代計量

Urinary Isoflavonoid Excretion as a Biomarker of Dietary Soy Intake during Two Randomized Soy Trials

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