MicroRNA-125a-3p Expression in Abdominal Adipose Tissues Is Associated with Insulin Signalling Gene Expressions in Morbid Obesity: Observations in Taiwanese

背景：微形核糖核酸（miRNA）在調節脂肪生合成及胰島素抗性扮演重要角色，但是肥胖者腹腔脂肪組織miRNA與胰島素訊息傳遞相關基因表現之間的相關性仍不確知。方法：本研究利用微陣列的平台來篩檢不同性別重度肥胖者腹腔脂肪組織中miRNA的表現，發現在腹腔網膜脂肪表現量最高的是miRNA-125a-3p，所以我們再用即時聚合酶鏈鎖反應確認並定量所有肥胖者（11位男性及10位女性）腹腔網膜脂肪中miRNA-125a-3p及胰島素訊息傳遞相關基因的表現，同時也以線性迴歸法分析miRNA-125a-3p與胰島素訊息傳遞相關基因表現及血中生化指標間的相關性。結果：肥胖男性腹腔網膜脂肪miRNA-125a-3p的表現較女性為高，腹腔皮下脂肪則無性別間之差異，有高miRNA-125a-3p表現量的肥胖男性，其c-Jun N-terminal kinase的基因表現量也會高，而胰島素接受器的表現量則較肥胖女性為低。miRNA-125a-3p與胰島素接受器及phosphatidylinositol 3-kinase的表現成負相關，而空腹血糖和膽固醇值與miRNA-125a-3p的表現則成正相關。這些相關性在肥胖男性很顯著，但在肥胖女性則不明顯。結論：本研究結果顯示miRNA-125a-3p參與了胰島素訊息傳遞路徑的調節，而腹腔網膜脂肪miRNA-125a-3p表現量增加可能是肥胖男性胰島素抗性的一個表徵。

關鍵字

重度肥胖；腹部脂肪組織；胰島素訊息路徑；胰島素抗性

並列摘要

Background: Micro (mi) RNAs have been found to play an important role in the regulation of adipogenesis and insulin sensitivity. However, associations between miRNA and insulin signalling-related gene expressions in abdominal adipose tissues in obese subjects remain unclear. Methods: We used a microarray platform to screen miRNA expressions in abdominal adipose tissues between genders in severely obese subjects and found that the top-ranking miRNA in abdominal omental adipose tissues was miRNA-125a-3p. MicroR-125a-3p and insulin signalling-related gene expressions in abdominal omental adipose tissues of all subjects (11 men and 10 women) were subsequently quantified by a real-time PCR. Also, associations of miR-125a-3p with insulin signalling-related gene expression and biochemical markers in obese subjects were analyzed by a linear regression analysis. Results: miR-125a-3p expressed by abdominal omental adipose tissues was much higher in obese men than women. No gender difference was observed in abdominal subcutaneous adipose tissues. Concomitant with high miR-125a-3p, c-Jun N-terminal kinase gene expression was also higher, whereas insulin receptor was lower in men than women. There were negative associations of miR-125a-3p with the insulin receptor and phosphatidylinositol 3-kinase expressions. Fasting plasma glucose and cholesterol levels were positively associated with miR-125a-3p expression. These associations were obvious in obese men but not women. Conclusion: Our results support the involvement of miR-125a-3p in regulating the insulin signalling pathway and imply that increased miR-125a-3p expression in omental adipose tissues may be a characteristic feature of insulin resistance in obese men.

並列關鍵字

morbid obesity ； abdominal adipose tissue ； miRNA-125a-3p ； insulin signalling pathway ； insulin resistant

參考文獻

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國際替代計量

MicroRNA-125a-3p Expression in Abdominal Adipose Tissues Is Associated with Insulin Signalling Gene Expressions in Morbid Obesity: Observations in Taiwanese

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