Expression of Cytokeratins in the Lining Epithelium and Ghost Cells of Calcifying Odontogenic Cyst

鈣化源性囊腫內襯上皮和鬼細胞之細胞角質表現

王逸平(Yi-Ping Wang) ； 王拯宗(Jeng-Tzung Wang) ； 劉步遠(Bu-Yuan Liu) ； 余權航(Chuan-Hang Yu) ； 陳皇旭(Huang-Hsu Chen) ； 孫安迪(Andy Sun) ； 江俊斌(Chun-Pin Chiang)

《中華牙醫學雜誌》 23卷2期 (2004/04) Pp. 89-96

https://doi.org/10.7105/CDJ.200404.0089

摘要

鈣化齒源性囊腫（COC）是一種罕見的齒源性囊腫，COC最特別之處在於內襯上皮中可發現鬼細胞。關於COC內襯上皮和鬼細胞之細胞角質表現之免疫組織化學研究之結果尚有爭議。而且部分學者所應用之抗體，通常含有同時對抗數種細胞角質之抗，因此對COC內襯上皮和鬼細胞中，到底含有那些特定細胞角質，常有解釋上之困難。本研究利用免疫組織化學染色技術，研究４例COC內襯上皮和鬼細胞之泛細胞角質（pan CK）、細胞角質10（CK10）、細胞角質13（CK13）、細胞角質14（CK14）和細胞角質19（CK19）之表現。免疫組織化學染色結果，依據陽性染色細胞之百分比，以半定量方法表示，其中０％，表示陰性染色；1-25％細胞陽性染色，表示局部陽性染色（＋）；26-75％細胞陽性染色，表示部分陽性染色（＋＋）；76-100％細胞陽性染色，表示均勻陽性染色（＋＋＋）。我們的研究結果發現COC內襯上皮細胞對pan CK和CK19之免疫染色，呈現部分（＋＋）或均勻（＋＋＋）陽性染色；對CK 14之免疫染色，呈現局部（＋）或部分（＋＋）陽性染色；對CK 10和CK 13之免疫染色，呈現陰性（－）染色。而COC中鬼細胞對pan CK和CK 19之免疫染，呈現均勻（＋＋＋）陽性染色；對CK 13和CK 14之免疫染色，呈現局部（＋）陽性染色；對CK 10之免疫染色，呈現陰性（－）染色。COC內襯上皮中有CK 14和CK 19之表現，表示COC內襯上皮細胞具有皮膚或口腔基底細胞和齒源性上皮細胞之特性。而COC鬼細胞中有CK 13、CK 14和CK 19之表現，表示COC中鬼細胞具有非角化多層鱗狀上皮細胞和齒源性上皮細胞之特性。

關鍵字

鈣化齒源性囊腫 ； 細胞角質 ； 鬼細胞 ； 內襯上皮

並列摘要

Calcifying odontogenic cyst(COC) is a rare type of odontogenic cyst. The most characteristic feature of COC is the presence of ghost cells (GS) in the lining epithelium. Previous immunohistochemical studies on the expression of cytokeratins in the lining epithelial cells (LEC) and GC of COC showed controversial results. In addition, several investigators used antibodies that recognize a mixture of cytokeratins in their studies, resulting in a difficulty in understanding what specific cytokeratins are present in the LEC and GC of COC. In this study, we used an immunohistochemical technique to investigate the expression of pan cytokeratins (pan CK), cytokeratin 10(CK 10), cytokeratin 13(CK1 3), cytokeratin 14(CK 14), and cytokeratin 19(CK 19) in the LEC and GC of 4 cases of COC. The immunostaining results were expressed in a semiquantitative fashion according to the estimated percentage of positively stained cells: 0%, negative (-); 1-25%, focally positive(+); 26-75%, variably positive(++); and 76-100%, uniformly positive(+++). We found that the LEC of COC were variably (++) or uniformly positive(+++) for pan CK and CK19, focally(+) or variably positive(++) for CK 14, and negative(-) for CK 10 and CK 13. The GC of COC were uniformly positive (+++) for pan CK and CK 19, focally positive(+) for CK 13 and CK 14, and negative (-) for CK 10. The expression of CK 14 and CK 19 in the LEC of COC indicates that the LEC of COC have skin or oral basal and odontogenic epithelial cell properties, respectively. Furthermore, the presence of CK 13, CK 14 and CK 19 in the GC of COC suggests that the GC of COC possess the specific CKs originally existing in non-keratinizing stratified squamous and odontogenic epithelial cells.

並列關鍵字

calcifiying odontogenic cyst ； cytokeratin ； ghost cell ； lining epithelium