本土蟲生線蟲之採集及對黃條葉蚤田間防治效果評估

Collection of Native Entomopathogenic Nematodes and Evaluation its Efficiency for Controlling of Phyllotreta striolata (Coleoptera: Chrysomelidae) on Crucifera Field

李平全(Ping-Chuan Lee)

《高雄區農業改良場研究彙報》 10卷2期 (1999/06) Pp. 12-21

https://doi.org/10.6958/RBKDAIS.199906.0012

摘要

利用釣蟲法在高屏地區採樣，採集到本土蟲生線蟲共六種，分別以代號PT-729、PT-825、PT-119、PT-210、PT-211、PT-212（學名未鑑定）。經室內測試以PT-729條件較佳，因此以該種線蟲作材料進行一系列之試驗。三種人工飼料作體外繁殖試驗，結果以脫脂大豆粉+類固醇+酵母粹取物+蔬菜油之配方產量最佳。以蛭石（2號）混合土壤保濕劑ASP加水30倍為儲藏與保護配方，在室溫經二十天之存活率為100%。在小白菜及青江白菜園作田間防治黃條葉蚤試驗，結果顯示，分別於整地前3天和播種時各處理一次本土蟲生線蟲，結果最佳，防治率約在71－84%。4月份之南台灣溫度較12月高，故4月份之防治效果較差。本土蟲生線蟲混合蘇力菌（Bacillus thuringiensis）噴灑於土壤中對黃條葉蚤之防治並無增加效果。由試驗結果顯示本土蟲生線虫具有防治地下害蟲之潛力。

關鍵字

本土蟲生線蟲 ； 黃條葉蚤 ； 體外培養

並列摘要

There are six kinds of entomopathogenic nematodes namely: PT-729, PT-825, PT-119, PT-210, PT-211, PT-212 collected from Pingtung and Kaohsiung areas. Among them, PT-729 was the best species used as material in this experement. For the mass production of PT-729, the mixture of soybean powder ＋yeast extract＋ vegetable oil was the best artificial diet. It was storaged at room temperature in vermiculite (No.2) mixing with the soil moisture agent ASP diluted with distilled water 30 times for 20 days being 100% survival rate. The PT-729 suspension, sprayed at both 3 days before land preparation and sowing day, had a good efficiency, proximately 71-84% of control rate against Phyllotreta striolata. The control rate in December was higer than in April due to temperature raising after April in southern Taiwan. PT-729 suspension with B. T. did not increase the control rate of P. striolata. The native entomopathogenic nematode PT-729 served as microbiological control agent for soil's insect pests has a great potential in the future.

並列關鍵字

Native entomopathogenic nematode ； Phyllotreta striolata ； Vitro culture