Shoot Regeneration and Polyacetylene Production in Root Cultures of Lobelia Erinus

林大楨(Ta-Chen Lin) ； 林鳳蘭(Feng-Lang Lin) ； 田中章江(Norie Tanaka) ； 石丸幹二(Kanji Ishimaru)

《The Chinese Pharmaceutical Journal》 53卷2期 (2001/04) Pp. 49-54

https://doi.org/10.7019/CPJ.200104.0049

並列摘要

Adventitious shoots of Lobelia erinus (Campanulaceae) were directly regenerated from the root tissues cultured in hormone-free Murashige-Skoog (MS) medium under lighted conditions. The regenerants proliferated well both in vitro (in hormone-free MS solid medium) and in vivo (in soil in a pot). The final survival frequency of the transplanted regenerants in soil was 90 %. There were no morphological differences between the regenerants and the parental plants. The polyacetylene (lobetyol, lobetyolin and lobetyolinin) content of the regenerants in vitro was identical to that obtained from shoot cultures regenerated from the callus cultures. The polyacetylene content of the regenerants cultivated in a pot was also identical with the field-grown parental plants. Direct shoot regeneration from L. erinus root tissues provides a good culture system for the micropropagation of this important medicinal herb.

並列關鍵字

Lobelia erinus ； Campanulaceae ； Polyacetylene ； Regeneration ； Root cultures ； Adventitious root