生化需氧量感測系統之研發

Development of a Biochemical Oxygen Demand Sensing System

蔣志德(Chih-Te Chiang) ； 周楚洋(Chu-Yang Chou)

《農業機械學刊》 15卷1期 (2006/03) Pp. 37-54

https://doi.org/10.30062/JAM.200603.0004

摘要

本研究是利用三極式電化學偵測器、活性污泥菌種及流動注射分析系統建立一生化需氧量(BOD)的感測系統，以檢測廢水之生化需氧量。
感測器採用白金工作電極及Ag/AgCl參考電極，其中白金工作電極用20μL的Nafion薄膜做表面修飾，以減少雜訊。活性污泥置於以壓克力製成的反應槽中心一18mm(L)×2mm (W)×2mm(D)的凹槽，樣本利用上述電極及流動注射分析系統在此區域產生反應並進行檢測。試驗時分別建立以人工廢水GGA(Glucose-glutamic acid)及豬糞尿廢水為樣本的標準曲線，從輸出的電流與時間的關係圖，利用終點法(End point)、固定時間法(Constant time)、斜率法Ⅰ (Quasi-kinetic Ⅰ)及斜率法Ⅱ (Quasi-kinetic Ⅱ)等四種方式推估BOD的濃度。
為驗證本系統之效能，利用現場取樣的豬糞尿廢水作測試，結果顯示採用GGA建立的標準曲線所測得的BOD濃度，以固定時間法以及斜率法Ⅰ的結果較佳，其檢測的誤差分別為9.0%以及7.7%。而採用豬糞尿廢水建立的標準曲線所測得的BOD濃度，以終點法、固定時間法及斜率法Ⅰ可以更準確地預估實際廢水之BOD值，其誤差分別為0.8%、2.5%及1.7%。

關鍵字

生化需氧量 ； 生物感測器 ； 三極式電化學偵測器 ； 流動注射分析系統

並列摘要

A biochemical oxygen demand (BOD) sensing system using the combination of three-electrode electrochemical detector, seed obtained from the activated sludge, and flow injection analysis system (FIA) was developed in this study to examine the BOD of wastewater.
The detector contained an Ag/AgCl electrode (Reference electrode, RE) and a Pt electrode (Working electrode, WE), which was coated with 20 μL Nafion to reduce noises. The reactor was fabricated with acrylic plastic, and the activated sludge was placed in an 18 mm (L)×2 mm(W)×2 mm (D) cave, in which the tested sample was reacted and detected with the above electrodes through the flow injection analysis system. Standard curves were built from the experimental data for both GGA (Glucose-glutamic acid) synthetic wastewater and swine wastewater by different approaches: end-point, constant-time, quasi-kinetic Ⅰ, and quasi-kinetic Ⅱ, respectively.
To verify the efficacy of this system, wastewater collected from pig farm was tested. The results showed that, when using the standard curves generated by GGA wastewater, the constant-time and quasi-kinetic Ⅰ had better performance in estimating the BOD concentration, with the deviations of 9.0% and 7.7%, respectively. In addition, when using the standard curves generated by the swine wastewater, the end-point, constant-time and quasi-kinetic Ⅰ had much better estimate than the above, with the deviations of 0.8%, 2.5%, and 1.7%, respectively.

並列關鍵字

Biochemical oxygen demand ； Biosensor ； Three-electrode electrochemical detector ； Flow injection analysis system