In this study, we use mass production of electrospinning process to make the nanofiber membrane, and we can obtained in the four different working hours to obtain the samples, the fiber average diameter is 275nm were observed by SEM. Further known by the ANOVA analysis of this mass production process in the long times to work, no significant differences in diameter of the nanofiber. In addition, from the water filtration test results shows that the nanofiber membrane can effectively filter solids size greater than 1000nm and from ANOVA analysis on this process in the long times to work, the nanofiber membrane of filtration capacity (SS values) no significant difference too. Finally, in the large width of the mass production of the electrospinning process, we have also achieved a stable and long time to collect the working conditions.