玻璃體手術時切除之視網膜上膜細胞培養研究

本文旨在對增殖性玻璃體視網膜病變(proliferative vitreoretinopath，PVR)與黃斑皺褶病例手術切除的視網膜上膜(pre-retinal membrane)的細胞性狀進行體外研究。將切除的膜片用切塊貼皿法接種於培養皿。用加有30%胎牛血清的F12培養液進行培養。對部分培養作了免疫細胞化學研究以鑑定細胞種類。在12例PVR中，10例有細胞從膜片伸出(83.3%)，其中5例細胞能增殖至融合(41.7%)。根據形態學及免疫細胞化學研究，從膜片長出的細胞中有視網膜色素上皮細胞(RPE)，神經膠質細胞和巨噬細胞。次代培養中的細胞基本上均為RPE。對12例PVR進行追訪，發現有細胞增殖的5例，每例都有視網膜剝離復發；無明顯細胞增殖的7例，僅1例復發。黃斑皺褶2例均未見細胞從膜片長出。本項工作顯示PVR病例膜片內的細胞（主要是RPE）具有較活躍的增殖能力。細胞增殖能力與手術預後有一定關係。

關鍵字

無資料

並列摘要

The purpose of the present study was to investigate in vitro behaviors of the cellular components of surgical excised preretinal membranes from patients with proliferative vitreoretinopathy (PVR) and macular pucker. Surgical excised membranes were plated in culture dishes by an explant method and cultured with F12 medium supplemented with 30% fetal bovine serum. Some cultures were studied with immunocytochemical methods to identify the cell types. In 12 PVR patients, 10 cases exhibited cell outgrowth from the membranes (83.3%), cells proliferated to confluence in 5 cases (41.7%). Based on morphological and immunocytochemical study, retinal pigment epithelial cells (RPE), glial cells, and macrophages were identified in the outgrowth from these membranes. In subcultures, virtually all cells were RPE. Follow-up study of 12 PVR patients showed that in 5 cases with cell proliferation, all had relapse of retinal detachment; in 7 cases without cell proliferation, only 1 case had relapse. No cell outgrowth was observed in 2 cases of macular pucker. The present study indicates that some cells in membranes from PVR patients, predominantly RPE, some exhibited active proliferation. The growth capacity of the cells in culture exhibited a relationship with the prognosis after surgery.

並列關鍵字

proliferative vitreoretinopathy ； cell culture ； preretinal membrane ； macular pucker

國際替代計量

玻璃體手術時切除之視網膜上膜細胞培養研究

全文下載

主題瀏覽