Simultaneous Determination of Resveratrol Derivatives in Vitis thunbergii Plant by High Performance Liquid Chromatography

葡萄科植物中resveratrol衍生物之高效液相層析定量研究

林傳福(Chwan-Fwu Lin) ； 陳建志(Chien-Chih Chen) ； 沈建昌(Chien-Chang Shen) ； 崔之華(Chih-Hua Chui) ； 黃鈺玲(Yu-Ling Huang)

《藥物食品分析》 20卷2期 (2012/06) Pp. 495-500

https://doi.org/10.6227/jfda.2012200210

並列摘要

The roots of Vitis thunbergii Sieb. Zucc. (Vt-R) are well-known bone-protective medicinal material and rich in active resveratrol derivatives, as revealed in our previous studies. The present study used five compounds, including four isolated ones, ampelopsin C, (+)-vitisin A, (-)-vitisin B, and (+)-e-viniferin, and one purchased resveratrol, as standards to establish an HPLC method in order to simultaneously determine these contents in the sample materials. The analyses method was validated and was performed by using a reverse-phase column (Cosmosil 5C18-AR-II) with absorbance detection at 280 nm. The results showed that ampelopsin C, (+)-vitisin A and (-)-vitisin B were the major three constituents in Vt-R and their contents were 3.57, 2.99, and 1.69 mg/g, respectively. Meanwhile, resveratrol gave a level of 0.33 mg/g, which was much lower than those three major constituents. To determined the contents of five standards in the stems and leaves of this plant, except for (+)-e-viniferin, the levels of ampelopsin C, (+)-vitisin A, (-)-vitisin B, and resveratrol were 1.13, 0.27, 0.57, and 0.14 mg/g, respectively, in the stems, which were much less than those in root part. However, five compounds were not detected in leaves. This analytic method was also employed to investigate the qualities of five piece-packed raw materials (Vt-1-Vt-5) of root of V. thunbergii collected from the herb market. The levels of ampelopsin C, (+)-vitisin A, and (-)-vitisin B were in the range of ND-0.35, ND-1.10, and ND-1.20 mg/g, respectively, which were markedly less than those in Vt-R. These five compounds in other six plants of Vitaceae were also investigated, including V. vinifera L., V. kelungensis Moriyama,Ampelopsis brevipedunculata (Maxim.) Traut., V. thunbergii var. taiwanianaLu, Cissus pteroclada Hayata, and C. repens Lam. As a result, the former three plants were found to contain all of five standards. Except for resveratrol, other four compounds were not detected in V. thunbergii var.taiwaniana and C. repens. None of five compounds was found in C. pteroclada.

並列關鍵字

Vitis thunbergii Sieb. & Zucc. ； Vitaceae ； ampelopsin C ； (+)-vitisin A ； (-)-vitisin B ； (+)-e-viniferin ； resveratrol ； HPLC

參考文獻

Huang,Y. L., Tsai, W. J., Shen, C. C. and Chen, C. C. 2005. Resveratrol derivatives from the roots of Vitis thunbergii. J. Nat. Prod. 68: 217-220.

Chiou, W. F., Shen, C. C., Chen, C. C., Lin, C. H. and Huang, Y. L. 2009. Oligostilbenes from the roots of Vitis thunbergii. Planta Med. 75: 856-859.

Huang, Y. L., Loke, S. H., Hsu, C. C. and Chiou, W. F. 2008. (+)-Vitisin A inhibits influenza A virus-induced RANTES production in A549 alveolar epithelial cells through interference with Akt and STAT1 phosphorylation. Planta Med. 74: 156-162.

Ku, K. T., Huang, Y. L., Huang, Y. J. and Chiou, W. F. 2008. Miyabenol A inhibits LPS-induced NO production via IKK/IKappa B inactivation in RAW 264.7 macrophages: possible involvement of the p38 and P13K pathways. J. Agric. Food Chem. 56: 8911-8918.

Wang, C. K., Chen, L. G., Wen, C. L., Hou, W. C., Huang, L. F., Yen, S. J., Shen, Y. J., Lin, S. Y. and Liang, Y. C. 2010. Neuroprotective activity of Vitis thunbergii var. taiwaniana extracts in vitro and in vivo. J. Med. Food 13: 170-178.