Several strains of Trichoderma spp. isolated from rhizosphere soils and rhizoplane of agricultural crops in Taiwan were selected to detect the root growth of bitter gourd, loofah, and cucumber. Some strains of tested species of Trichoderma in this study promoted the root growth of these cucurbitaceous crops as compared with untreated plants. Tests in greenhouse showed the strains of the Trichoderma species significantly increased of 26 to 61 % in seedling height, 85-209% in root exploration, 27 to 38% in leaf area, and 38 to 62% in root dry weight for 15 days after sowing seeds of bitter gourd. Similarly, These Trichoderma strains also increased seedling growth of loofah and cucumber. In a test of chlorophyll concentration, the results indicated that the concentration of chlorophyll (mg/cm2 of leaves) was significantly increased in the plants treated with Trichoderma. Based on regression tests, there was no relationship between percentage of root colonization of Trichoderma and enhanced plant growth.