Early Stages of Infection of Lily Leaves by Botrytis elliptica and B. cinerea

灰黴病菌Botrytis elliptica與B. cinerea 於百合葉片之感染初期

候秉賦(Ping-Fu Hou) ； 陳昭瑩(Chao-Ying Chen)

《植物病理學會刊》 12卷2期 (2003/06) Pp. 103-108

https://doi.org/10.6649/PPB.200306_12(2).0005

摘要

據所觀察，Botrytis elliptica 感染葵百合(Oriental bybrid cv. Star Gazer)葉片造成壞疽病斑；B.cinerea則僅在葵百合葉片上造成針狀斑點。本研究即在比較二灰黴病菌於葵百合葉片上早期的感染情形。接種後12小時，B.elliptica與B.cinerea孢子在葵百合葉背上的發芽率達95%；B. elliptica的附著器形成率則明顯地高於B. cinerea。然而，在接種後24小時，百合受B. elliptica的侵入率可達54.5%；B. cinerea則無法成功地侵入葵百合葉片組織。在B. elliptica及B. cinerea嘗試侵入的部位，可見死亡的葵百合葉片細胞，有些並呈現螢光。B. elliptica之嘗試侵入可能阻於此等死亡細胞；但有些感染菌絲則繼續生。此外，B. elliptica之侵染並在葵百合葉片造成螢光物質的累積；但接種B. cinerea的葵百合葉片則無類似的反應。

關鍵字

灰黴病菌 ； 感染 ； 百合 ； 植物反應

並列摘要

According to our observations, infection of lily leaves (Oriental hybrid cv. Star Gazer) by Botrytis elliptica resulted in the development of necrotic lesions, but inoculation with B. cinerea only caused tiny specks. A comparative study was conducted herein to investigate early stage of infection of 'Star Gazer' leaves by these pathogens. Microscopical examination 12 hr after inoculation showed that the percentage of germinated conidia of both B. elliptica and B. cinerea on the abaxial surfaces of lily leaves reached to 95%, but the number of appressoria produced by the germ tubes of B. elliptica was significantly higher than that produced by B. cinerea. In addition, the penetration of lily leaves by B. elliptica was nearly 54.5%, but it was nil by B. cinerea. During the attempted penetration by B. elliptica and B. cinerea, some of the epidermal cells at penetration sites became dead. Among these dead cells, some were associated with fluorescence. Although some of the penetration hyphae of B. elliptica were retarded by the dead cells of lily leaf epidermis, some still expanded and resulted in the development of necrotic lesion. Fluorescent depositions as indicated by aniline blue staining appeared in lily leaf tissues infected by B. elliptica. However, this phenomenon was not observed in lily leaves inoculated with B. cinerea.

並列關鍵字

Botrytis ； infection ； lily ； plant response