Effect of L-Arginine and Its Analogues on the Hypersensitive Response of Tobacco (Nicotiana tabacum L. cv. TT-5) Induced by Avirulent Ps61(superscript -) Strain of Ralstonia solanacearum

精氨酸與其結構類似物對無毒力青枯病菌(Ralstonia solanacearum)Ps61(上标 -)菌株在菸草所誘發過敏性反應之影響

周俊吉(Chun-Chi Chou) ； 李敏惠(Miin-Huey Lee) ； 曾國欽(Kuo-Ching Tzeng) ； 曾德賜(Dean Der-Syh Tzeng)

《植物病理學會刊》 15卷2期 (2006/06) Pp. 97-105

https://doi.org/10.6649/PPB.200606_15(2).0004

摘要

在以無毒力青枯病菌(Ralstonia solanacearum)Ps61()菌株行人工接種菸草(Nicotinana tabacum cv.TT-5)植株的同時，精氨酸(L-arginine)的添加對所誘發的過敏性反應具有明顯的促進作用。此一促進現象明顯與添加濃度有關，其病徵表現明顯為接種細菌的作用所造成；精氨酸的影響在添加濃度達1mM以上即可明顯看出，與未添加處理之對照組比較，其主要可明顯提高過敏性反應壞疽病徵的嚴重程度。壞疽病徵的加速，顯示由接種細菌Ps61菌株所誘發的主動防衛反應有受到促進的現象，然此並非其對病原毒害作用的強化，在反應過程巾，精氨酸的添加對Ps61菌株在寄主體內族群的衰減與對照處理比較並無明顯差異。相對於精氨酸添加的促進作用，添加包括甲基精氨酸(NMA)、硝基精氨酸甲基酯(NAME)與刀豆氨基酸(canavanine)等精氨酸結構類似物,以及methyl-guanidine或aminoguanidine等亞氨基單二胺類(guanidine)複合物，均可使此一過敏性反應壞疽病徵的顯現大為延遲與減輕，其中並以刀豆氨基酸與aminoguanidine的添加抑制效果最為明顯，而精氨酸的添加則可回復刀豆氨基酸添加所顯現的抑制效果。由試驗中所見精氨酸的添加對過敏性反應的明顯促進作用，以及不同精氨酸結構類似物與亞氨基單二肢類複合物的添加對病徵表現所顯現的差異性抑制效果顯示，在供試菸草之過敏性反應中，誘導型一氧化氮自由基生合成酶的參與作用值得進一步瞭解。

並列摘要

An L-arginine enhancement effect on hypersensitive response (HR) was observed in tobacco (Nicotinana tabacum cv. TT-5) challenge-inoculated with an avirulent Ps61 strain of Ralstonia solanacearum. The enhancement effect was dosage dependent, and symptom expression appeared to be a function of the challenging bacteria. The effect of arginine became prominent at concentration greater than 1mM, which was manifested by the development of earlier and severer necrotic HR symptoms compared to that of the controls. The accelerated necrogenic response reflected the functioning of a promoted active defense triggered by the challenging bacterium. However, there was no indication of increased toxigenic effect since the rate of population decline of Ps61 during the course remained nearly unchanged when treated with L-arginine. In contrast to the arginine promoting effect, the application of arginine analogues (NMA, NAME or canavanine), and guanidino compounds (methyl-guanidine or aminoguanidine), greatly retarded and reduced the necrotic response. Canavanine and aminoguanidine were among them the most effective, whereas the supplementation of L-arginine reversed the inhibitory effect of canavanine. The observed enhancement effect of arginine and the differential inhibitory activity of arginine analogues and guanidine compounds suggested that the role of an inducible nitric oxide synthase may play a role in the studied HR in tobacco.

並列關鍵字

L-arginine ； hypersensitive reaction ； nitric oxide