胡瓜嵌紋病毒（CMV）相關之沙漠玫瑰輪斑嵌紋病

Cucumber mosaic virus-Associated Ringspot Mosaic Disease of Desert Rose

張怡珊(Y. S. Chang) ； 林郁瑋(Y. W. Lin) ； 陳宗祺(T. C. Chen) ； 陳煜焜(Y. K. Chen)

《植物病理學會刊》 21卷1期 (2012/03) Pp. 39-45

https://doi.org/10.6649/PPB.201203_21(1).0005

摘要

2010年於嘉義地區發現葉片具有嵌紋、黃化輪紋與線條等病癥的沙漠玫瑰﹝Desert rose, Adenium obesum （Forssk.） Roem. & Schult, Family Apocynaceae﹞。罹病組織粗汁液內具有直徑約28nm的疑似球形病毒顆粒。血清學反應顯示與胡瓜嵌紋病毒（Cucumber mosaic virus, CMV）有正相關。單離後的病毒株可機械接種於指示植物並引起與CMV感染後之類似病徵。以專一性引子對配合反轉錄-聚合酶連鎖反應（RT-PCR）增幅CMV RNA 3靠3’端半段（即RNA4）的cDNA。所增幅的cDNA經選殖與解序已取得基因資料庫登錄碼（GenBank accession number）AB667971。基因序列分析顯示外鞘蛋白基因的核苷酸與胺基酸序列與CMV第一亞群及第二亞群的對應序列分別有92.2~95.0%和75.5~77.7%相同度。利用番茄斑萎病毒屬的廣效簡併式引子對並未能在罹病的沙漠玫瑰檢測得番茄斑萎病毒屬的病毒感染，顯示CMV是沙漠玫瑰輪斑嵌紋病相關的主要因子。

關鍵字

胡瓜嵌紋病毒 ； 番茄斑萎病毒 ； 沙漠玫瑰 ； 黃化輪紋 ； 嵌紋

並列摘要

Desert rose [Adenium obesum (Forssk.) Roem. & Schult, family Apocynaceae] with symptoms of mosaic, chlorotic ringspots and line patterns on leaves were observed in Chiayi area in 2010, Spherical virus particles with a diameter of approximately 28 nm were observed in crude sap prepared from symptomatic leaves. Serological tests indicated that the virus reacted positively to a rabbit antiserum prepared to Cucumber mosaic virus (CMV). Isolated virus culture was mechanically transmissible to indicator plants and induced symptoms similar to those incited by CMV. The cDNA of the 3'-half of CMV RNA 3 has been obtained in reverse transcription-PCR with specific primers. The amplified cDNA fragment was cloned and sequenced (GenBank Accession No. AB667971). Nucleotide sequences of the coat protein open reading frame (CP ORF) had 92.2 to 95.0% and 75.5 to 77.7% sequence identity to those of CMV in subgroups I and II, respectively. Tospoviruses were not detected from infected plants of desert rose by using wide-spectrum degenerated primers for Tospovirus in RT-PCR indicating the CMV is possibly the main factor associated to the ringspot mosaic disease of desert rose.

並列關鍵字

Cucumber mosaic virus ； Tospovirus ； desert rose ； chlorotic ringspot ； mosaic