臺灣北部十字花科作物鏈格孢菌屬分離株之菌落特徵及種群鑑定

Colony Characteristics and Species-Group Identification of Alternaria Isolates from Crucifers in Northern Taiwan

張佲(山韋)(M. W. Chang) ； 陳昭瑩(C. Y. Chen)

《植物病理學會刊》 22卷4期 (2013/12) Pp. 367-380

https://doi.org/10.6649/PPB.201312_22(4).0005

摘要

本研究自臺灣北部十字花科作物黑斑病病葉及蔬菜種子分離鏈格孢菌屬真菌，將經單孢分離及鏈格孢菌屬專一性引子對增幅Alt a I基因之195 bp DNA片段的菌株，依V8培養基上之菌落特徵區分為A、B、C及D四型。在無傷口下接種切離葉片，只有C型菌落之鏈格孢菌屬菌株可以造成典型病斑。以AIt-for/AIt-rev引子對增幅此四型菌株之Alt a I同源序列（含195 bp Alt a I DNA片段）作序列比對及群集分析，屬於A、B及D型菌落之鏈格孢菌屬分離株與Alternaria alternata Alt a I序列有95%相似度，依群集分析歸於alternata種群；而屬於C型菌落且具致病性之鏈格孢菌屬菌株與A. brassicicola Alt a l序列有100%相似度，歸於brassicicola種群。

關鍵字

十字花科作物 ； 鏈格孢菌屬 ； 群集分析 ； 親緣關係 ； 種群

並列摘要

In this study, Alternaria species were isolated from cruciferous leaves with black spot symptom and the vegetable seeds. Single spore isolates capable of amplifying 195 bp DNA fragment with Alternaria genus-specific primers from Alt a I gene were categorized into A, B, C and D types based on colony morphology. Pathogenecity test by inoculation on the detached leaves without wounding showed that only the Alternaria isolates with C type colony caused lesion development. Sequence alignment and cluster analysis of Alt a I homologous sequences amplified with AIt-for/Alt-rev primer pair indicated that the Alternaria isolates with A, B, or D type colony, sharing 95% homology with A. alternate Alt a I sequence, were grouped into 'alternata' species-group while the pathogenic Alternaria isolates with C type colony, sharing 100% homology with A. brassicicola Alt a I sequence, were grouped into 'brassicicola' species-group.

並列關鍵字

Crucifers ； Alternaria ； Alt a l ； cluster analysis ； phylogenetic relationship ； species-group