Characterization and Identification of Catharanthus Roseus Epigenetic-related Genes that in Response to Peanut Witches'-broom Phytoplasma-mediated Infection

日日春基因靜默機制相關基因參與花生簇葉病菌質體誘發花器葉化機制之探討

曾欣怡(Hsin-I Tseng) ； 林長平(Chan-Pin Lin) ； 林詩舜(Shih-Shun Lin)

《植物病理學會刊》 23卷1期 (2014/03) Pp. 67-77

https://doi.org/10.6649/PPB.201403_23(1).0006

摘要

ContigViews資料庫平台提供龐大的日日春植物（Catharanthus roseus）轉錄體基因資訊。為了研究表觀遺傳對於花生簇葉病植物菌質體（peanut witches'-broom phytoplasma; PnWB）誘發日日春花器葉化的影響。其參與在後轉錄時期基因靜默（post-transcriptional gene silencing; PTGS）及核醣核酸引發之去氧核醣核酸甲基化（RNA-directed DNA methylation; RdDM）相關基因已由此資料庫中選殖出來，並與阿拉伯芥同源基因相互比較。這些相關基因的演化關係及在健康花（HF）與PnWB誘發第四型（S4）葉狀花的表現量都被詳細的研究。研究結果顯示，日日春ARGONAUTE 5（CrAGO5）及RNA-DEPENDENT RNA POLYMERASE 1（CrRDR1）基因在S4葉狀花中表現量有明顯的增加。然而，大部份的PTGS及RdDM相關基因在HF及S4樣本中的表現量都維持恆定，顯示表觀遺傳的基因表現必須維持恆定。這些證據推測CrAGO5與CrRDR1可能參與在PnWB誘導之葉狀花及植物抗病反應。

關鍵字

次世代基因定序 ； 日日春 ； 花生簇葉病植物菌質體 ； 後轉錄時期基因靜默 ； 核醣核酸引發之去氧核醣核酸甲基化

並列摘要

The high-throughput transcriptome profile of Catharanthus roseus in the ContigViews database provides useful information on the interaction between phytoplasma and plant. In this study, several post-transcriptional gene silencing (PTGS)- and RNA-directed DNA methylation (RdDM)-related genes were identified in C. roseus, which might be involved in peanut witches'-broom (PnWB) phytoplasma-mediated leafy flower formation. The evolutionary relationships and expression profiles of these genes in healthy flowers (HF) and Stage 4 (S4) PnWB-infected leafy flowers were also characterized. Notably, ARGONAUTE 5 (CrAGO5) and RNA-DEPENDENT RNA POLYMERASE 1 (CrRDR1) in C. roseus were significantly up-regulated in S4 leafy flowers, whereas other PTGS- and RdDM-related genes maintained normal expression levels. These results suggested that CrAGO5 and CrRDR1 might involve in PnWB-mediated leafy flower formation and plant defense.

並列關鍵字

Next-generation sequencing ； Catharanthus roseus ； peanut witches'-broom phytoplasma ； post-transcriptional gene silencing ； RNA-directed DNA methylation