- 期刊
ENDOGLUCANASE PRODUCING POTENTIAL OF BACILLUS SPECIES ISOLATED FROM THE GUT OF LABEO ROHITA
摘要
In the current research, a microorganism, Bacillus aquimaris, had been isolated from the gut of Labeo rohita and classified by 16S ribosomal DNA genome sequencing. Sugarcane bagasse (substrate) was used for producing endoglucanase in submerged fermentation using this bacterium. Various factors affecting endoglucanase production were screened by Plackett-Burman design and optimization of significant parameters were carried out by the Box-Behnken design of response surface methodology. Results revealed that among nine parameters screened, concentrations of substrate, MgSO_4 and (NH_4)_2SO_4 were found significant. Maximum yield of endoglucanase (437.3833 IU) was achieved at 2.5% sugarcane bagasse, 0.01% MgSO_4 and 0.5% (NH_4)_2SO_4 after 24 h of the fermentation period. These results suggested the potential utilization of this strain for nutritional purpose to promote the growth of fishes for commercialization of aquaculture.
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延伸閱讀
- 黃湘燕（2016）。The study of keratinase from Bacillus amyloliquefaciens〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU201602996
- Wang, C. Y. (2009). Investigation of novel extremely halotolerant bacteria and polyglucan hydrolytic enzymes [doctoral dissertation, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU.2009.00354
- 宋君陵（2010）。Isolation and Characterization of α-Amylase Containing Bacteria (Exiguobacterium sp.)〔碩士論文，淡江大學〕。華藝線上圖書館。https://doi.org/10.6846/TKU.2010.00151
- Singh, A., Houpt, E., & Petri, W. A. (2009). Rapid Diagnosis of Intestinal Parasitic Protozoa, with a Focus on Entamoeba histolytica. Interdisciplinary Perspectives on Infectious Diseases, 2009(), 144-151. https://doi.org/10.1155/2009/547090
- 曾瑋盈（2007）。Partial Characterization of Bacteriocin by a Newly Isolated Bacillus subtilis〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2007.10383