The research studied 5 male and 5 female badminton players who were back-up players in the Badminton Team of National Cheng Kung University. The biochemical examination of blood urine (BUN), hemoglobin (HB), blood lactate (BLA) and urinary protein were recorded before and after two training lessons at a training day. The data was to probe if the plan of the training load (training intensity and training volume) was appropriate and also to probe if the players’ physical condition was good. All the data was analyzed by one-way ANOVA. The conclusions of this study are described as follows: 1. The training intensity is obviously not enough for the training lessons twice a training day. 2. The training volume is appropriate twice a training day. 3. The players’ physical condition did not totally recover and was not ideal.