透過您的圖書館登入
IP:3.233.221.42
  • 期刊

耐力訓練對骨骼肌肌纖維類型與細胞凋亡關係的影響

Effect of Endurance Exercise Training on the Relationship between Myosin Heavy Chain Isoforms and Apoptosis in Skeletal Muscle

摘要


To examine the relationship between myosin heavy chain (MyHC) isoforms and apoptosis in the gastrocnemius and soleus after endurance exercise training. Methods: Male Sprague-Dawley rats were randomly assigned to an untrained (CON, n=6) or an endurance training group (ET, n=6). The ET exercised on a treadmill for 10 weeks. Results: After endurance exercise traning, citrate synthase activity was increased in both gastrocnemius and soleus (p<.05). In gastrocnemius, the content of MyHC I, MyHC IIa and MyHC IIx were increased while MyHC IIb was decreased (p<.05). In soleus, the content of MyHC I was increased while MyHC IIa was decreased (p<.05). Apoptotic index was decreased in both gastrocnemius and soleus. Also, the apoptotic index of soleus was lower than the gastrocnemius after endurance training (p<.05). B-cell leukemia/lymphoma 2 (Bcl2) mRNA was up-regulated while apoptosis inducing factor (AIF) mRNA was down-regulated in gastrocnemius of ET. Bcl-2, Bcl-2-associated X protein (Bax) and heat shock protein 70 (HSP 70) mRNA were up-regulated while caspase-9 and AIF mRNA were down-regulated in soleus of ET. Conclusions: 1) Decreased apoptosis and down-regulated the mRNA expression of mitochondrial dependent apoptotic signaling were correlated with that the content of MyHC I increased in both gastrocnemius and soleus after endurance exercise training that is more sensitive to influence soleus than gastrocnemius. 2) Endurance exercise training not only down-regulated the mRNA of mitochondrial dependent apoptotic signaling but up-regulated the mRNA of anti-apoptosis in soleus that didn't occur in gastrocnemius.

並列摘要


To examine the relationship between myosin heavy chain (MyHC) isoforms and apoptosis in the gastrocnemius and soleus after endurance exercise training. Methods: Male Sprague-Dawley rats were randomly assigned to an untrained (CON, n=6) or an endurance training group (ET, n=6). The ET exercised on a treadmill for 10 weeks. Results: After endurance exercise traning, citrate synthase activity was increased in both gastrocnemius and soleus (p<.05). In gastrocnemius, the content of MyHC I, MyHC IIa and MyHC IIx were increased while MyHC IIb was decreased (p<.05). In soleus, the content of MyHC I was increased while MyHC IIa was decreased (p<.05). Apoptotic index was decreased in both gastrocnemius and soleus. Also, the apoptotic index of soleus was lower than the gastrocnemius after endurance training (p<.05). B-cell leukemia/lymphoma 2 (Bcl2) mRNA was up-regulated while apoptosis inducing factor (AIF) mRNA was down-regulated in gastrocnemius of ET. Bcl-2, Bcl-2-associated X protein (Bax) and heat shock protein 70 (HSP 70) mRNA were up-regulated while caspase-9 and AIF mRNA were down-regulated in soleus of ET. Conclusions: 1) Decreased apoptosis and down-regulated the mRNA expression of mitochondrial dependent apoptotic signaling were correlated with that the content of MyHC I increased in both gastrocnemius and soleus after endurance exercise training that is more sensitive to influence soleus than gastrocnemius. 2) Endurance exercise training not only down-regulated the mRNA of mitochondrial dependent apoptotic signaling but up-regulated the mRNA of anti-apoptosis in soleus that didn't occur in gastrocnemius.

參考文獻


陳聰毅、林念璁(2008)。單次離心運動對骨骼肌粒腺體釋出細胞色素C及細胞凋亡誘發因子的影響。體育學報。41(4),31-42。
陳聰毅、林念璁(2006)。耐力運動訓練對誘發骨骼肌細胞凋亡之粒腺體訊息傳遞路徑的影響。體育學報。39(4),11-22。
Alessio, H. M.(1993).Exercise-induced oxidative stress.Medicine and Science in Sports and Exercise.25(2),218-224.
Andersen, J. L.,Schjerling, P.,Saltin, B.(2000).Muscle, genes and athletic performance.Scientific American.283(3),48-55.
Arslan, S.,Erdem, S.,Sivri, A.,Hascelik, Z.,Tan, E.(2002).Exercise-induced apoptosis of rat skeletal muscle and the effect of meloxicam.Rheumatology International.21(4),133-136.

延伸閱讀