To examine the relationship between myosin heavy chain (MyHC) isoforms and apoptosis in the gastrocnemius and soleus after endurance exercise training. Methods: Male Sprague-Dawley rats were randomly assigned to an untrained (CON, n=6) or an endurance training group (ET, n=6). The ET exercised on a treadmill for 10 weeks. Results: After endurance exercise traning, citrate synthase activity was increased in both gastrocnemius and soleus (p<.05). In gastrocnemius, the content of MyHC I, MyHC IIa and MyHC IIx were increased while MyHC IIb was decreased (p<.05). In soleus, the content of MyHC I was increased while MyHC IIa was decreased (p<.05). Apoptotic index was decreased in both gastrocnemius and soleus. Also, the apoptotic index of soleus was lower than the gastrocnemius after endurance training (p<.05). B-cell leukemia/lymphoma 2 (Bcl2) mRNA was up-regulated while apoptosis inducing factor (AIF) mRNA was down-regulated in gastrocnemius of ET. Bcl-2, Bcl-2-associated X protein (Bax) and heat shock protein 70 (HSP 70) mRNA were up-regulated while caspase-9 and AIF mRNA were down-regulated in soleus of ET. Conclusions: 1) Decreased apoptosis and down-regulated the mRNA expression of mitochondrial dependent apoptotic signaling were correlated with that the content of MyHC I increased in both gastrocnemius and soleus after endurance exercise training that is more sensitive to influence soleus than gastrocnemius. 2) Endurance exercise training not only down-regulated the mRNA of mitochondrial dependent apoptotic signaling but up-regulated the mRNA of anti-apoptosis in soleus that didn't occur in gastrocnemius.
To examine the relationship between myosin heavy chain (MyHC) isoforms and apoptosis in the gastrocnemius and soleus after endurance exercise training. Methods: Male Sprague-Dawley rats were randomly assigned to an untrained (CON, n=6) or an endurance training group (ET, n=6). The ET exercised on a treadmill for 10 weeks. Results: After endurance exercise traning, citrate synthase activity was increased in both gastrocnemius and soleus (p<.05). In gastrocnemius, the content of MyHC I, MyHC IIa and MyHC IIx were increased while MyHC IIb was decreased (p<.05). In soleus, the content of MyHC I was increased while MyHC IIa was decreased (p<.05). Apoptotic index was decreased in both gastrocnemius and soleus. Also, the apoptotic index of soleus was lower than the gastrocnemius after endurance training (p<.05). B-cell leukemia/lymphoma 2 (Bcl2) mRNA was up-regulated while apoptosis inducing factor (AIF) mRNA was down-regulated in gastrocnemius of ET. Bcl-2, Bcl-2-associated X protein (Bax) and heat shock protein 70 (HSP 70) mRNA were up-regulated while caspase-9 and AIF mRNA were down-regulated in soleus of ET. Conclusions: 1) Decreased apoptosis and down-regulated the mRNA expression of mitochondrial dependent apoptotic signaling were correlated with that the content of MyHC I increased in both gastrocnemius and soleus after endurance exercise training that is more sensitive to influence soleus than gastrocnemius. 2) Endurance exercise training not only down-regulated the mRNA of mitochondrial dependent apoptotic signaling but up-regulated the mRNA of anti-apoptosis in soleus that didn't occur in gastrocnemius.
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