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單次運動對間歇性低氧誘發心肌微管相關蛋白1輕鏈3-II的影響

Effects of a Single Bout of Exercise on Intermittent Hypoxia-Induced Microtubule-Associated Protein 1 Light Chain 3-Ii Expression in Rat Left Ventricle

摘要


目的:探討運動後不同休息時間對間歇性低氧誘發心肌自噬反應的影響。方法:將8週齡Sprague-Dawley雄性大鼠,隨機分成控制組(control, CON)、運動組(exercise, EXE)、間歇性低氧曝露組(intermittent hypoxia, IH)、空氣曝露組(room air, RA)、運動後1(post-exercise 1 h, PE1h)及3小時(PE3h)合併RA或IH組。大鼠給予單次持續8小時IH(氧氣濃度下降至2~6 %約1-2 秒/75 秒),單次運動方式以24 m/min速度、2%坡度之強度在跑步機上跑步60 min。動物犧牲後,檢測左心室心肌肝醣、自噬反應相關蛋白及粒腺體功能相關mRNA表現量。結果:運動後心肌細胞微管相關蛋白1輕鏈3-II(microtubule-associated protein 1 light chain 3-II, LC3-II)/LC3-I表現量、肝醣含量下降(p < .05),p62表現量沒有變化(p > .05)。IH對心肌p62表現量沒有影響(p > .05),與RA比較,IH之LC3-II/LC3-I、核內呼吸因子1和2(nuclear respiratory factor 1 and 2, NRF-1 和NRF-2)mRNA表現量上升(p < .05)。與IH比較,PE1h+RA、PE3h+RA 及PE3h+IH各組LC3-II/LC3-I及NRF-1和NRF-2 mRNA表現量均下降(p < .05),雖PE1h+IH之NRF-1和NRF-2 mRNA表現量下降(p > .05),但LC3-II/LC3-I表現量沒有改變(p > .05)。結論:IH曝露前3小時從事運動,可避免IH造成心肌LC3-II/LC3-I表現量上升的現象。

關鍵字

心臟 氧化壓力 活性氧物種

並列摘要


Purpose: Investigating the effects of different time intervals after exercise on intermittent hypoxia (IH)-induced autophagy in rat left ventricle. Methods: Eight-week-old male Sprague-Dawley rats were randomly assigned to control (CON), exercise (EXE), IH, room air exposure (RA), post-exercise 1 and 3 h combined with RA (PE1h + RA and PE3h + RA) or IH (PE1h + IH and PE3h + IH) groups. Rats were exposed to a single IH bout (2% - 6% O2 for 1-2 sec/75 sec) for 8 h and a single exercise bout on treadmill (24 m/min, 2% grade) for 60 min. Myocardial glycogen, autophagy-related proteins and mitochondrial-related mRNAs were determined after euthanisation. Results: Immediately after exercise, microtubule-associated protein 1 light chain 3-II (LC3-II)/LC3-I protein levels decreased (p < .05) but p62 levels did not alter (p > .05). As compared with RA, IH did not alter p62 levels but did increase LC3-II/LC3-I protein and nuclear respiratory factor 1 and 2 (NRF-1 and NRF-2) mRNA levels (p < .05). Compared with IH, significantly lower LC3-II/LC3-I protein and NRF-1 and NRF2 mRNA levels were found in PE1h + RA, PE3h + RA and PE3h + IH (p < .05). NRF-1 and NRF-2 mRNA levels were lower in PE1h+IH than in IH (p < .05), although LC-II/LC3-I levels were similar (p > .05). Conclusion: Exercise at 3 h before IH exposure could prevent IH-induced increased myocardial LC3-II/LC3-I levels.

參考文獻


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