Dedifferentiation Effect of hTERT on hMSCs and Thus Enhances Osteogenesis

人類終端轉錄酶經由去分化效應增強骨髓幹細胞骨分化之能力

陳俊利(Chun-Li Chen) ； 王振穎(Chen-Ying Wang) ； 洪士杰(Shih-Chieh Hung) ； 劉謙美(Chien-Mei Liu) ； 侯連團(Lein-Tuan Hou)

《臺灣牙周病醫學會雜誌》 15卷1期 (2010/03) Pp. 39-49

摘要

雖然人類間葉幹細胞較身體其他組織之已分化細胞擁有較快的增生速率與較好的分化能力，然而，細胞老化與自然分化的現象阻礙了人類骨髓幹細胞在培養狀態下的長期研究。此外，就人類間葉幹細胞骨生成之能力，此一在植體學與牙周再生學上至爲重要的課題來說，亦會被間葉幹細胞的老化與自然分化所嚴重影響。我們實驗的目標在於希望能克服間葉幹細胞老化與自然分化的難題，並經由回復人類間葉幹細胞的幹性而增加其骨生成之能力。

關鍵字

人類間葉幹細胞 ； 人類終端轉錄酶 ； 自然分化

並列摘要

Cellular senescence and spontaneous differentiation are critical phenomena that impair long-term study of human mesenchymal stem cells (hMSCs) in vitro, despite its high proliferation rate and multipotency comparing to those of differentiated cells derived from different tissues. In addition, bone formation capacity, which might be the most critical factor in implantology and periodontal regeneration, would also be severely influenced by cellular senescence and spontaneous differentiation of hMSCs. The present study was designed to overcome these problems and also explored to drive hMSCs toward stemness and thus to enhance osteogenesis.

並列關鍵字

Human mesenchymal stem cells ； hTERT ； spontaneous differentiation