台灣捐血者全面實施Mi^a抗原檢驗

Miltenberger血型抗原是東南亞常見的MNS血型的亞型之一，約佔2-7％，Miltenberger血型的異體抗體是台灣血庫作業中最常見的紅血球異體抗體，此類病人須要輸用Miltenberger抗原陰性的紅血球避免產生輸血反應及避免刺激異體抗體再次產生。目前尚無適合用於大量篩檢捐血者的市售抗血清試劑。我們利用融合瘤技術，自人類B淋巴球培養出能分泌效價穩定anti-Mi^a單株抗體的融合瘤細胞株，此單株抗體可鑑定Miltenberger血型中重要的Mi^a抗原，同時應用於血型分析儀（PK7300）進行台灣捐血者全面檢驗Mi^a抗原，並將Mi^a抗原結果標示於紅血球血品標籤。如此，醫院血庫作業中可直接挑選Mi^a抗原陰性的紅血球血品進行輸血前交叉作業，降低病人產生溶血性輸血反應、增進輸血安全。

關鍵字

Miltenberger血型； Mi^a抗原；捐血；融合瘤技術

並列摘要

Miltenberger, a family of blood groups, is the result of gene recombination leading to encoding a hybrid molecule of glycophorin A and B. The occurrence of Miltenberger blood group is relatively high (2-7%) at Southeast Asia region. In Taiwan, antibodies to Miltenberger blood group have been found to be the most frequent red cell alloantibody with clinical significance. Evidence showed that transfusion of GP. Mur-negative RBC to patients with previously detectable anti-＂Mi^a＂ could decrease the incidence of hemolytic transfusion reaction and antibody boosting. Since the complexity of Miltenberger blood group antigens and the lack of commercial antiserum suitable for applying on Automated Microplate Analyzer, it is difficult in large-scale screening of these antigens in blood donors. By using hybridoma technology, we have established an anti-Mi^a-producing cell line from human B lymphocytes. This monoclonal antibody specifically recognized Mi^a antigen on red blood cell surface, and shows stable agglutination reactions that can easily be detected by PK7300 Automated Microplate System. Currently, we have detected all blood donors for Mi^a antigen and labeled the test results on red blood cell components. This facilitates the selection of Mi^a-antigen-negative RBC in blood bank routines, and thus lower the hemolytic transfusion reactions and improve the safety of blood transfusion.

並列關鍵字

miltenberger blood groups ； Mi^a antigen ； blood donation ； hybridoma technology

參考文獻

Tippett P, Reid ME, Poole J, et al: The Miltenberger subsystem: is it obsolescent? Transfus Med Rev 1992;6:170-82. doi: 10.1016/S0887-7963(92)70167-9

Lin M, Broadberry RE: Immunohematology in Taiwan. Transfus Med Rev 1998;12:56-72. doi: 10.1016/S0887-7963(98)80090-4

Broadberry RE, Lin M: The distribution of the MiⅢ(GP. Mur) phenotype among the population of Taiwan. Transfus Med 1996;6:145-8. doi: 10.1046/j.1365-3148.1996.d01-64.x

Lin M, Broadberry RE: An intravascular hemolytic transfusion reaction due to anti-‘Mia' in Taiwan. Vox Sang 1994;67:320. doi: 10.1159/000462625

Wu KH, Chang JG, Lin M, et al: Hydrops foetalis caused by anti-Mur in first pregnancy--a case report. Transfus Med 2002;12:325-7. doi: 10.1046/j.1365-3148.2002.00394.x

國際替代計量

台灣捐血者全面實施Mi^a抗原檢驗

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