A method named as ”instantaneous close collection of ammonia volatilized from soil” was used to study the effects of nitrogen fertilizer forms, applying depth and mixture of N fertilizer and P fertilizer on N loss by volatilization from a manual loessial soil. The results obtained show that the different forms of N fertilizers had different volatilization amount. Urea was the largest in ammonia volatilization followed by ammonium sulphate and ammonium bicarbonate. The placement of fertilizers had great influence on volatilization with N loss being reduced by 27.7% and 13.3%, respectively ,when fertilizers were placed in 6~12 cm and 0~6 cm layer as compared with surface application. The mixture of applying N fertilizer with superphosphate had a significant effect on protecting ammonia volatilization for ammonium bicarbonate and this effect increased with increase of superphosphate ammount in the mixture.