The objective of this study was to develop an environmental control technology in acclimatization chamber for grafted seedlings. The nonlinear control methodology with the Condition-Sequence control implementation was adopted as the main control scheme for the system Logic control circuits finished by industrial wiring elements were created to model the tempera, humidity and CO2 control processes. The system was designed for both flexible adjustments to environmental conditions and easy operations for users. Regarding environmental control, if the weather conditions (temperature and relative humidity) are appropriate for grafted seedling curing, the ambient air is directed to regulate the chamber environment in order to save energy. Otherwise, vents in the chamber will be closed to form a close system, and the inner environmental control will be made. Tow control strategies, high-low limit and on-off & time delay control action, were adopted as the main control methodology for the system. Results indicate that the control system can satisfactorily achieve expected goals for providing suitable curing conditions and modulating the environmental factors such as temperature, relative humidity, illuminance and CO2 for grafted seedlings.