蛋內接種伏馬鐮孢毒素對鴨胚發育之影響

Effects of Fumonisin Inoculation on Duck Embryo Development In ovo

鄭永祥(Yeong-Hsiang Cheng) ； 張銘煌(Ming-Haung Chang) ； 趙智宇(Chi-Yu Chao)

《宜蘭技術學報》 4期 (2000/06) Pp. 97-104

https://doi.org/10.6179/nit.2000.04.19

摘要

本研究之目的旨在探討，不同濃度伏馬鐮孢毒素（fumonisin B1; FB1）蛋內接種後對鴨胚發育之影響。試驗使用200枚菜鴨受精蛋，逢機分配於5組，每處理二重複，每重複10顆蛋，分別於蛋內接種0、20、40、80或160μg/egg，純化之FB1，接種用FB1分別溶於160μl二次蒸餾水並經過濾滅菌（稀釋液），而對照組者僅接種稀釋液。試驗一，蛋於孵化第1日接種，試驗期間記錄死亡率及接種後14日時之體測性狀。試驗二則在孵化第14日接種，記錄死亡率及接種後7日時之體測性狀。試驗結果顯示死亡率會隨著時間及劑量而增加，試驗一中以20μg、40μg、80μg 或160μg FBl接種者，其死亡率分別為10%、20%、20%及100%；而在第14日接種者，除40及160μg FB1有10%死亡率，其餘均無死亡。在體測性狀方面，僅試驗一中20μg接種組有顯著較長之頸長。無論是第1日或第14日的40μg以上接種者，鴨胚之肝、腎及心臟均可觀察到組織病理學變化。

關鍵字

鴨胚 ； 伏馬鐮孢毒素 ； 蛋內接種

並列摘要

This study was design to investigate the different concentration of Fumonisin B, (FB1) on duck embryo development after in ovo inoculation. There were 200 fertile eggs of Tsaiya being used, and alloted randomly into five treatment groups as follows 0, 20, 40, 80 or 160μg/egg. FB1, was dissolved in 160μI of double-distillated water and sterilization by millipore filtration, and control group was inoculated with sterilized water only. In experiment 1, fertile eggs were inoculated at day one, the mortality and the 14th day old body performance were recorded. In experiment 2, eggs were inoculated at 14th day old, and the mortality and the body performance was recorded at the 7th day after inoculation.In experiment 1, the results showed that the mortality was increased along with dose and time dependent manner. The rate of mortality were 10%, 30%, 30% and 100% for 20, 40, 80 or 160μg/egg, respectively. However, there were 10% mortality for 40 and 160μg/egg and the other doses were hatched appearance normally in experiment 2. The histopathology alteration of liver, kidney and heart was observed, after above 40μg inoculated, both in experiment 1 and experiment 2.

並列關鍵字

Duck embryo ； Fumonisin ； in ovo