利用簡單重複序列標記建立台灣主要稉稻品種的鑑別技術

Variety Identification among Major Japonica Rice Cultivars of Taiwan Based on Simple Sequence Repeat Markers

謝廉一(Lien-Yi Hsieh) ； 吳岱融(Dai-Rong Wu) ； 胡凱康(Kae-Kang Hwu)

《植物種苗》 9卷2期 (2007/06) Pp. 25-39

https://doi.org/10.30077/SN.200706.0003

摘要

為鑑別台灣地區的主要稉稻品種，本研究利用46個簡單重複序列標記對25個參試品種（系）進行分析。在經過聚合酵素連鎖反應及毛細管電泳後，其中20組引子在參試品種之中具有多型性，共21基因座，平均對偶基因數為2.90、PIC值為0.38。除台稉11號及台稉15號的分子標記多型性模式完全相同之外，其餘品種（系）均可被最少6個標記完全鑑別。將品種鑑定的目標限定為具有相當栽培面積的18個品種，則除了台稉11號與台稉15號之外，其餘品種（系）均可被最少4個標記完全鑑別。配合選用長度範圍有差異的標記，將各標記PCR增幅產物混合後進行毛細管電泳分析，將可有效的降低分析所需的時間與分析成本。

關鍵字

稉稻品種 ； 品種鑑別 ； 簡單重複序列 ； 主成分分析

並列摘要

In order to identify the major Japonica type rice cultivars grown in Taiwan, 46 simple sequence repeat (SSR) markers were screened against 25 rice cultivars. After polymerase chain reaction (PCR) and capillary electrophoresis, 20 SSR markers showed polymorphism among cultivars tested. In total, there were 21 polymorphic loci, with average 2.90 alleles per locus, averaged Polymorphic Information Content is 0.38. All cultivars can be distinguished based on polymorphic SSR markers, except Taikeng No. 11 and Taikeng No. 15. The minimum set of markers required to distinguish all 25 entries composed of 6 markers. If the varieties subject to identification are restricted to 18 major cultivars which are still in use, only 4 markers are needed to identify all but the two previously mentioned cultivars. By choosing markers with non-overlapping allele sizes, PCR products may be analyzed by multiplex capillary electrophoresis and thus greatly reduce the time and cost to conduct the analysis.

並列關鍵字

Japonica rice cultivar ； variety identification ； simple sequence repeat ； principal coordinate analysis