以簡單重複序列標記分析水稻品種內之遺傳均一性

Genetic Uniformity within Rice Cultivars Measured by Simple Sequence Repeat Markers

吳岱融(Dai-Rong Wu) ； 胡凱康(Kae-Kang Hwu)

《植物種苗》 9卷4期 (2007/12) Pp. 1-16

https://doi.org/10.30077/SN.200712.0001

摘要

本研究使用20組簡單重複序列分子標記對6個水稻品種進行品種內遺傳均一性調查，共偵測到21個基因座，其中18個基因座在品種間表現具多型性，11個基因座在品種內表現多型性。品種內異質性來源主要有兩種，一為同一基因座內不同類型的同型合子，二為異型合子，以前者占絕大部分。經主成分分析與集群分析結果顯示，參試6個水稻品種內依然存在不同程度的異質性，但不影響品種間的區分。本文除討論品種內異質性對於品種檢定的影響，並建議以遺傳距離0.1作為品種內異質性的上限。

關鍵字

品種鑑定 ； 品種內異質性 ； 簡單重複序列標記 ； 主成分分析 ； 集群分析

並列摘要

The genetic uniformity of six rice cultivars was assessed by 20 SSR markers in this study. In all 21 loci detected, 18 and 11 loci were polymorphic between and within cultivars, respectively. There were two types of non-uniformity within cultivars, the overwhelming one was the type that had more than one homozygote in a locus, and the other one was heterozygote. Results of principal coordinates analysis and cluster analysis showed that the cultivars were uniquely identifiable in spite of the non-uniformity detected within each cultivars. Based on the level of non-uniformity obtained in this research, we recommended using 0.1 as the acceptable upper limit for averaged genetic distance among variants within a cultivar. The effects of genetic non-uniformity based on molecular markers on variety identification and Plant Breeder's Right were also discussed.

並列關鍵字

variety identification ； genetic uniformity ； simple sequence repeat ； principal coordinates analysis ； cluster analysis