模擬消化試驗對血管張力素轉換酶抑制物（鮪魚蒸煮汁水解物）活性之影響

Imitative Digestion Tests on the Activity of Angiotensin I-Converting Enzyme Inhibitor - Hydrolysate Derived from Tuna Cooking Juice

黃至盛(Jyh-Sheng Hwang) ； 柯文慶(Wen-Ching Ko)

《臺灣農業化學與食品科學》 42卷4期 (2004/08) Pp. 299-305

https://doi.org/10.6578/TJACFS.2004.039

摘要

本研究以具降血壓功能之鮪魚蒸煮汁水解物OAH(蛋白酶Orientase酵素水解物在此簡稱OAH)為原料，應用不同分段的豬小腸酵素萃取液及市售腸胃消化酵素進行消化解試驗，探討OAH對血管張力素轉換酶(angiotensin I-converting enzyme, ACE)的抑制活性經腸道中勝肽酶水解後之變化。結果顯示均分為五個分段的豬小腸黏膜上的總蛋白酶活性(total proteolytical activity, TPA)不同：前段及中間豬小腸的TPA值較尾端高，且十二指腸、空腸、迴腸的TPA值依序遞減，故蛋白質在的前段及中間部分的消化吸收較多。OAH經胃蛋白酶(pepsin)水解(pH3、37℃)後，ACE抑制活性上升(IC50值下降)，然而，再經豬小腸各分段的酵素液水解，發現均使ACE抑制活性下降；而且隨著作用時間愈長，下降幅度越大；其中以第一段豬小腸粗酵素液作用後之ACE抑制活性下降最多，12小時作用後水解物之ACE抑制活性僅剩原有活性的62%(IC50值由9.65 mg/mL升高為17.15 mg/mL)，其次為第二段、第三段、第四段、第五段。以上試驗結果顯示，OAH經過胃消化後ACE抵制活性將增加，但到達腸道中由於消化酵素的作用後則明顯下降；胰蛋白質酶(trypsin)或胰凝乳蛋白酶(chymotrypsin)的作用會增加ACE抑制勝肽的形成，進而增加OAH對ACE的抑制功能。

關鍵字

鮪魚蒸煮汁 ； 消化試驗 ； 血管張力索轉換酶 ； 胜肽

並列摘要

The Orientase hydrolysate (OAH), derived from tuna cooking juice and possessing antihypertensive effect, was applied to digestion tests with enzymes from different segments of porcine small intestine of with gastrointestinal enzymes to investigate the effects of digestive enzymes on ACE-inhibiting activity of OAH. The results indicated that the total proteolytical activity (TPA) in the mucosa of five consecutive segments of intestine were different. Higher TPA values were found in the proximal and medial segments, and TPA values declined from duodenum, jejunum to ileum. It is obvious that much hydrolysis and absorption of proteins take place in the proximal and medial regions of the small intestine. Enhanced ACE inhibitory activity (decreased in IC50 value) was observed when OAH was digested with pepsin at 37℃, pH 3.0. However, after peptic predigestion followed by hydrolysis with enzymes from different intestine segments, all OAH preparations exhibited evident reduction in ACE inhibitory activity. Moreover, the longer digestive time showed the lower ACE inhibitory activity. Among these digestions, the enzymes from the first intestine segment strongly reduced ACE inhibitory activity. OAH remained only 62% of the original activity, and IC50 values increased from 9.65 to 17.15 mg/mL, after digested by the first intestine segment for 12 h. The effect of lowering ACE inhibitory activity along the gut decreased from the first through the fifth segment. These results suggest that the ACE-inhibiting effect of OAH increased by the digestive enzymes from stomach, and declined by intestinal enzymes. Additionally, hydrolysis performed with trypsin or chymotrypsin will conduct the release of ACE inhibitory peptides and increase ACE-inhibiting effect in OAH.

並列關鍵字

Tuna cooking juice ； Digestion test ； Angiotensin I-converting enzyme ； Peptide