犬螺旋桿菌檢查技術的比較

Comparison of Diagnostic Methods of Gastric Helicobacter-like Organism in Dogs

陳燕萍(Yen-Ping Chen) ； 張本恆(Peng-Heng Chang) ； 連于欣(Yu-Hsin Lien) ； 曾文怡(Wei-Yi Tseng) ； 劉金鳴(Ching-Ming Liu) ； 黃慧璧(Hui-Pi Huang)

《臺灣獸醫學雜誌》 32卷3期 (2006/09) Pp. 217-224

https://doi.org/10.7009/TVJ.200609.0217

摘要

本研究的目的為比較犬胃螺旋樣桿菌（gastric Helicobacter-like organisms, GHLO） 的診斷方式，並探討GHLO與胃組織病理變化之間的相關性。五十隻臨床上無消化道症狀的犬參與本研究，在進行採樣前二週無投與任何藥物。實驗為利用胃內視鏡進行胃黏膜組織的生檢採樣，分別進行抹片細胞學、組織病理學與聚合脢鏈鎖反應檢查。抹片細胞學、組織病理學及聚酶鏈鎖反應診斷GHLO的陽性率，分別是68%、66%與60%，此三種診斷方法的結果在統計上無顯著差異，三種診斷方法的總陽性率為76%。犬GHLO細菌量的多寡與胃組織炎症細胞浸潤的程度在統計上無相關性，胃組織浸潤的細胞以淋巴球和漿細胞為主。聚合酶鏈鎖反應結果顯示16S rRNA核酸序列和H. felis、H. salamonis、H. bizzozeronii、H. heilmannii、H. bilis與H. pylori等菌種（species）間的相似性高達95%以上，而且與其他屬別（genus）無相似性。

關鍵字

犬 ； 診斷方式 ； 螺旋桿菌 ； 盛行率

並列摘要

The purposes of present study were to compare different diagnostic methods, smear cytology, histopathology and polymerase chain reaction, for detecting gastric Helicobacter-like organism (GHLO), and to clarify the relationship between GHLO and histopathologic changes. Fifty dogs without any episode of gastrointestinal signs, or without administering medicine 2 weeks prior to biopsy were included in the study. The gastric biopsy specimens were obtained from the mucosa of the cardia, fundus and pylorus using endoscopic forceps. The specimens were examined by smear cytology, histopathology and polymerase chain reaction (PCR). The GHLOs were found in 68%, 66%, and 60% of the dogs by smear cytology, histopathology, and PCR respectively. No statistically significant differences were found in 3 diagnostic methods in detecting GHLO. The over-all prevalence of GHLO in the present study was 76%. No correlation was found between the degree of GHLO colonization and infiltration of inflammatory cells. Lymphoc ytes and plasma cells were the major inflammatory cells. The DNA sequences of PCR products revealed the selected partial 165 rRNA sequences shared more than 95% similarity with various Helicobacter spp., including H. felis, H. salomonis, H. bizzozeronii, H. heilmannii, H. bilis and H. pylori. The sequences were not compatible to any other genus of organisms.

並列關鍵字

Canine ； diagnostic methods ； gastric Helicobaeter-like organism ； prevalence