無菌鼠自發性病變回溯性分析

Retrospective Study of Spontaneous Lesions in the Germfree Mice and Rats

卓宜興(Yi-Xing Zhuo) ； 梁鍾鼎(Chung-Tiang Liang) ； 林裕翔(Yu-Hsiang Lin) ； 陳錦富(Jin-Fu Chen) ； 曾彥勳(Yen-Shun Tseng) ； 廖秀鈴(Hsiu-Ling Liao) ； 梁善居(Sun-Chi Liang) ； 黃彥智(Yen-Te Huang)

《臺灣獸醫學雜誌》 35卷2期 (2009/06) Pp. 124-130

https://doi.org/10.7009/TVJ.200906.0124

摘要

統計分析國家實驗動物中心從2004年1月至2007年12月無菌鼠送檢例行性健康監測共計168隻，包括Narl: Swiss Webster小鼠53隻、C57BL/6JNarl小鼠38隻、BALB/cByJNarl小鼠33隻、Tac: N (SD)大鼠44隻。經病理診斷發現共有96隻無菌鼠於組織病理學上有明顯異常，共計發生有16種自發性病變，其中有10隻無菌鼠發生有兩種以上的自發性病變。無菌小鼠常見病變包括視網膜變性佔19.4% (24/124)、心外膜／心肌礦物質沉積佔10.5%(13/124)、唾液腺淋巴組織增生佔7.3% (9/124)與腎病佔6.5% (8/124)；無菌大鼠主要病變以水腎病50.0% (22/44)與肺泡組織細胞症13.6%(6/44)最為常見。其他病變包括肺異物性肉芽腫、肺腫瘤、腎小管礦物質沉積、心肌病、嗜酸性晶體性肺炎、肝腫瘤、血管瘤與動脈周圍炎等散發病例共有25例。分析結果發現，無菌鼠較常發生的病變與特定病原鼠相似，多半為品系背景病變與自發性病變。此外，分析中發現100%(168/168)的無菌鼠，均出現有盲腸膨大症候群及腸道培耶氏斑發育不全，此兩種現象為無菌鼠之背景特徵，可能是無菌鼠缺乏正常腸道菌叢協助腸道內容物分解消化及刺激腸道培耶氏斑發育所致。

關鍵字

盲腸膨大症候群 ； 腸道培耶氏班發育不全 ； 自發性病變 ； 無菌小鼠 ； 無菌大鼠

並列摘要

A total of 168 germfree rodents including 53 Nan: Swiss Webster mice, 38 C57BL/6JNarI mice, 33 BALB/cByJNarl mice, and 44 Tac: N (SD) rats were necropsied and microscopically examined at the National Laboratory Animal Center in the period of 2004 to 2007. Sixteen different types of spontaneous lesions were observed in 96 rodents and 10 out of these 96 animals were identified with more than one type of lesions. The most frequently observed lesions in mice were retinal degeneration (19.4%, 24/124), epicardial/cardial mineralization (10.5%, 13/124), salivary gland perivascular lymphoid hyperplasia (7.3%, 9/124) and nephrosis (6.5%, 8/124), and in rats were hydronephrosis (50.0%, 22/44) and alveolar histiocytosis (13.6%, 6/44). Other less frequent lesions included foreign body granuloma in lung, lung tumor, kidney with tubular mineralization, cardiomyopathy, acidophilic macrophage pneumonia, liver tumor, hemangioma and periarteritis observed in 25 rodents. The results indicated that the most common spontaneous lesions observed in the germfree rodents were likely related to heredity, similar to occurrence of spontaneous lesions noted in the specific pathogen free (SPF) rats and mice. All the 168 (100%) germfree rodents had enlarged cecum syndrome and hypoplastic Peyer's patches that were background characteristics in the germfree rodents due to the absence of microflora originated.

並列關鍵字

enlarged cecum syndrome ； hypoplasia of peyer's patches ； spontaneous lesions ； germfree mice ； germfree rats