Detection of Residues by Use of Solid-Phase Extraction and Reversed Phase High-Performance Liquid Chromatography after Oral Administration of Amoxicillin in Bass Muscle

本試驗為進行安默西林在養殖鱸魚體內的藥物動力學及殘留性研究，並建立適當的停藥期作為養殖使用劑量與使用期間的參考。藥物動力學實驗以加州鱸每日口投20、40 mg/kg魚體重為劑量，連續投藥五日，殘留之安默西林以固相萃取及高效液相層析法進行分析。使用Mightysil RP-18 GP 250-4.6 mm φ 5μm液相層析管柱，流動相為0.005 M KH2PO4 (pH 6.8)：Methanol (90:10)，流速1 mL/min，並以紫外光偵測器監測210 nm波長的吸收，每件檢體的分析時間小於15分鐘。而以本分析方法所建立之安默西林標準品檢測濃度區間為0.01~10 μg/g，魚肉添加安默西林的檢測濃度為0.04~10μg/g，平均回收率78%。魚肉中安默西林之偵測極限(LOD) 為0.04μg/g，定量極限(LOQ)為0.1μg/g。由藥物動力學試驗可得，加州鱸在連續五日口服投予安默西林，於停藥後6小時(T(下標 max)可達魚體肌肉中藥物濃度的高峰值(C(下標 max)) 0.84μg/g (投藥量20 mg/kg b.w.) 及1.18μg/g (投藥量40 mg/kg b.w.)，而距最後投藥後72小時的安默西林殘留濃度，則低於本分析方法的檢測極限。因此建議養殖加州鱸以40 mg/kg 魚體重的安默西林投藥量連續使用五日，則停藥期應為最後投藥後五日。

關鍵字

安默西林；鱸魚；藥物動力學；殘留；高效液相相層析

並列摘要

An experimental trial was performed to establish an adequate depletion period of amoxicillin in bass. The amount of amoxicillin was applied by oral administration at the dose of 20 and 40 mg/kg body weight, and was given at a daily dose for five consecutive days. Amoxicillin levels were determined using a solid-phase extraction and high-performance liquid chromatography (HPLC) method. The drug was eluted from Mightysil RP-18 GP 250-4.6 mm φ5 m column with a mobile phase consisting of 0.005 M KH2PO4 buffer with pH 6.8-MeOH (90:10, v/v), at a flow rate of 1 mL/min. The effluent was monitored using a UV detector set at 210 nm. Each analysis required no longer than 15 mm. The validated concentration ranges of this method were 0.01 μg/mL to 10 μg/mL for working standard solutions, and 0.04 to 10 μg/g for muscle. Mean absolute recoveries were 78%. The limits of detection (LCD) and limits of quantitation (LOQ) for bass muscle were 0.04 and 0.1 quantitation g/g, respectively. After 5 consecutive oral doses of amoxicillin, residues in 6-h-postdosing (T(subscript max)) bass were at the maximum 0.84 and 1.18 μg/g (C(subscript max)) toward 20 and 40 mg/kg b.w. dosage, residue levels after a 72-h depletion decreased to below the methods limit of quantitation after last oral treatment. At the dosage (40 mg/kg b.w.), we suggested that the recommended withdraw period was 5 days.

並列關鍵字

amoxicillin ； drug residues ； pharmacokinetics study ； bass muscle ； HPLC

國際替代計量

Detection of Residues by Use of Solid-Phase Extraction and Reversed Phase High-Performance Liquid Chromatography after Oral Administration of Amoxicillin in Bass Muscle

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