The Significances of Antiglobulin Test in Dogs with Immune-Mediated Hemolytic Anemia

庫姆氏試驗在免疫性溶血性貧血患犬之臨床意義

陳琬筑(Wan-Chu Chen) ； 鄭謙仁(Chian-Ren Jeng) ； 蘇璧伶(Bi-Ling Su)

《臺灣獸醫學雜誌》 35卷4期 (2009/12) Pp. 233-240

https://doi.org/10.7009/TVJ.200912.0233

摘要

我們建立了臨床可例行性檢測的庫姆氏試驗(AT)，用以確診犬免疫性溶血性貧血。本報告收集27隻臨床上利用靜脈注射人類免疫球蛋白作為治療的患犬，評估AT試驗在罹患免疫性溶血性貧血患犬隻之臨床意義。其中66.7%為AT試驗陽性。抗體種類的分析上，50%為IgG陽性，5.6%IgM陽性，27.8%為IgG及IgM陽性，5.6%為IgG及補體C3陽性，以及11.1%為IgG, IgM和C3皆為陽性。我們進一步分析此試驗之結果與貧血的程度以及預後之相關性，發現在個別的病例中AT試驗抗體力價越高，貧血程度越嚴重。然而抗體的種類及陽性結果與預後並無直接相關。總之，AT力價可反應臨床症狀之改善及血液檢查之結果，不過單次AT力價檢查結果無法準確的評估癒後。

關鍵字

庫姆氏試驗 ； 犬 ； 免疫溶血性貧血

並列摘要

In this study, we made canine antiglobulin test (AT) in Taiwan as a routine diagnostic procedure of canine IMHA, collected 27 IMHA dogs treated with 1g/kg intravenous human immunoglobulin (hIVIG), evaluated the significance of AT in canine IMHA. The positive ratio of IMHA dogs was 66.7% (18/27). The pattern of antisera classes was 50% were IgG positive, 5.6% were IgM positive, 27.8% were positive for both IgG and IgM, 5.6% were positive for both 1gM and C3, and 11.1% were positive for IgG, IgM, and C3. We analyzed the association between AT and severity of anemia; and between AT and prognosis. The results showed AT titers variation would affect the severity of the anemia reversely in different cases individually. However the results and antisera classes pattern of AT could not be used as a prognostic indicator. We concluded that although AT would affect clinical and laboratory examinations of IMHA patients, it could not predict the prognosis.

並列關鍵字

antiglobulin test ； dog ； immune-mediated hemolytic anemia