There are numerous pathogens including virus, bacteria, fugal and parasite may cause respiratory disease in reptile. In tortoise, respiratory infection is a common and important clinical disease, especially in captive animals which are poor of husbandry and improper environment. This case is a red-footed tortoise present respiratory distress. Trachal pulmonary lavage by trans-tracheal washing was performed under a sterile procedure. Sample was send to (prepared for) cytology, fungal culture, bacteria culture, antibiotic sensitivity test, mycoplasma PCR and herpesvirus PCR detection. Only one (strain of) bacteria, Stenotrophomonas maltophilia, was isolated from sample test. Stenotrophomonas maltophilia was reported as pathogen cause (ing) snakes and crocodiles pathological change, but in tortoises was not yet reported (in tortoises).