［簡訊］靜脈注射kisspeptin誘發犬隻血中排卵素之變化

Kisspeptin是由KiSS-1基因（黑色素瘤轉移抑制基因）轉譯出的蛋白，又可以被蛋白水解酶裂解成kisspeptin-14、kisspeptin-13、kisspeptin-10等多種衍生物，這些衍生物統稱為kisspeptins在許多動物之胎盤、肝臟、小腸、胰臟、脾以及周邊淋巴血液組織可偵測到此－蛋白的表現。當kisspeptin與其細胞膜上的受體GPR54結合時，可調控某些癌細胞的生長和轉移，近年來更發現具有影響生殖內泌素分泌及調節哺乳動物生殖生理的功能。目前已知牛羊等動物可經周邊血管給予kisspeptin後促進下視丘分泌GnRH來誘發LH和FSH的分泌，進而刺激性腺生成相關一生內泌素。本研究中我們將kisspeptin經由靜脈注射的方式給予去勢犬隻，藉由檢測其血中LH濃度的變化，以期能了解犬隻對於kisspeptin的劑量與時程反應效果結果顯示靜脈注射25ng/kg劑量的kisspeptin可有效誘發犬隻LH之分泌，使得血中LH濃度顯著上升，且連續注射此劑量的kisspeptin也能連續誘發LH的分泌。因此推測kisspeptin對於大隻刺激激性腺素的分泌具有正向的調控。

關鍵字

犬； kisspeptin ；排卵素

並列摘要

Kisspeptin, a product of KiSS-1 gene, also known as metastin, can be degraded into several derivatives including Lisspeptin-14, kisspeptin-13 and Lisspeptin-i0. These derivatives are also called kisspeptins. Kisspeptin can be detected in many organs including the embryo, liver, intestine, pancreas, spleen and peripheral lymphoid tissues in different kinds of animals. It has been discovered that kisspeptin regulates the growth and metastasis of tumor cells when conjugates with its GPR 54 receptor on the cell membrane. Recently, it has also been stated that kisspeptin regulates reproductive endocrine productivity. Ovine and bovine research showed that IV-injected kisspeptin significantly stimulated the release of follicle stimulating hormone (FSH) and luteinizing hormone (LH). In order to realize the effect of kisspeptin on LH change, neutered dogs were given kisspeptin intravenously and serum LH concentrations were then measured at different time points. The results showed that 25 ng/kg of kisspeptin induced LH secretion effectively and resulted in an elevation in blood LH concentration. Continual injection of the same dose of kisspeptin also showed a time-course effect with continually elevated LH concentration. The results suggest that kisspeptin possesses a positive function in regulation of gonadotropin secretion in dogs.

並列關鍵字

dog ； kisspeptin ； luteinizing hormone

國際替代計量

［簡訊］靜脈注射kisspeptin誘發犬隻血中排卵素之變化

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