Bovine herpesvirus type I (BHV-1) causes acute febrile respiratory disease in cattle, which is also known as infectious bovine rhinotracheitis (IBR). Bovine ephemeral fever virus (BEFV) also causes acute febrile respiratory disease mainly associated with pulmonary emphysema and edema. Infections caused by these two viruses in domesticated herds lead to great economic losses to the dairy farmers. In this study, BHV-1 and BEFV viruses were amplified in cultured MDBK (Maden Darby bovine kidney cells) and BHK (baby hamster kidney cell) cells, inactivated by BEI (binary ethylenimine), and formulated with water-in-oil-in-water adjuvant. No adverse reactions were observed in guinea pigs and cattle immunized with this vaccine. The titer of serum antigen-specific antibody in immunized cattle was significantly (p<0.01) higher than that of the control group 8 weeks post primary vaccination and remained high for 12 months. In the challenge study conducted in guinea pigs, animals in the immunized group had a higher lymphocyte proliferation index than those in the control group and were protected from the challenge. Our results indicated that this vaccine formulation is effective to elicit both humoral and cell-mediated immune responses against BHV-1 and BEFV.
Bovine herpesvirus type I (BHV-1) causes acute febrile respiratory disease in cattle, which is also known as infectious bovine rhinotracheitis (IBR). Bovine ephemeral fever virus (BEFV) also causes acute febrile respiratory disease mainly associated with pulmonary emphysema and edema. Infections caused by these two viruses in domesticated herds lead to great economic losses to the dairy farmers. In this study, BHV-1 and BEFV viruses were amplified in cultured MDBK (Maden Darby bovine kidney cells) and BHK (baby hamster kidney cell) cells, inactivated by BEI (binary ethylenimine), and formulated with water-in-oil-in-water adjuvant. No adverse reactions were observed in guinea pigs and cattle immunized with this vaccine. The titer of serum antigen-specific antibody in immunized cattle was significantly (p<0.01) higher than that of the control group 8 weeks post primary vaccination and remained high for 12 months. In the challenge study conducted in guinea pigs, animals in the immunized group had a higher lymphocyte proliferation index than those in the control group and were protected from the challenge. Our results indicated that this vaccine formulation is effective to elicit both humoral and cell-mediated immune responses against BHV-1 and BEFV.
為了持續優化網站功能與使用者體驗,本網站將Cookies分析技術用於網站營運、分析和個人化服務之目的。
若您繼續瀏覽本網站,即表示您同意本網站使用Cookies。