水生病原菌對雜交吳郭魚(Oreochromis Niloticus×Oreochromis Mossambicus)頭腎來源之巨噬細胞非特異性免疫反應之影響

Non-Specific Immune Responses of Hybrid Tilapia (Oreochromis Niloticus×Oreochromis Mossambicus) Anterior Kidney-Derived Macrophage against Different Aquatic Bacteria

陳明輝(Ming-Hui Chen) ； 洪紹文(Shao-Wen Hung) ； 林育興(Yu-Hsing Lin) ； 鄭清福(Ching-Fu Cheng) ； 涂青宇(Ching-Yu Tu) ； 張鎮璿(Chen-Hsuan Chang) ； 王孟亮(Min-Liang Wong) ； 王渭賢(Way-Shyan Wang)

《臺灣獸醫學雜誌》 37卷3期 (2011/09) Pp. 203-213

https://doi.org/10.7009/TVJ.201109.0203

摘要

實驗目的在於研究吳郭魚頭腎來源巨噬細胞對水生病原菌的非特異性免疫反應。由實驗結果顯示經誘發之頭腎巨噬細胞的吞噬能力，以吞噬yeast之能力最高，吞噬Streptococcus iniae、Vibrio sp.及Edwardsiella tarda之能力最低。頭腎巨噬細胞吞噬Vibrio sp.後可產生最高的自由基量。此外，在細菌接種12小時內，頭腎巨噬細胞一氧化氮之產生和細胞死亡即有顯著地增加，其中以吞噬Vibrio sp.最高。利用FITC-Annexin V和propidium iodium進行染色後再以flow cytometer進行檢測，顯示在接種細菌12小時後，頭腎巨噬細胞發生apoptosis的比例顯著增加，其中以接種活的Vibrio sp.最高。綜合結果發現不同水生病原菌確實會影響頭腎巨噬細胞的非特異性免疫反應能力，並以apoptosis為主要誘發巨噬細胞死亡之機制。

關鍵字

細胞計畫性死亡 ； 自由基 ； 巨噬細胞 ； 非特異性免疫 ； 吳郭魚

並列摘要

The aim of this study was to investigate the non-specific immuno-responses of hybrid tilapia (Oreochromis Niloticus×Oreochromis Mossambicus) anterior kidney-derived macrophage (AKM) against different aquatic bacteria. Our results showed that AKM possessed a highest phagocytic ability to engulf yeast, but a lowest phagocytic ability to engulf Streptococcus iniae, Vibrio sp., and Edwardsiella tarda. Furthermore, AKM showed the highest production of free radicals when engulfing Vibrio sp.. The production of nitric oxide, cell death, and apoptosis stained with FITC-annexin V and propidium iodide were significantly increased in AKM treated with bacteria for 12 h. AKM exhibited the highest apoptosis rate when AKM were inoculated with Vibrio sp.. Based on these results, aquatic bacteria could affect non-specific immune responses of AKM and induced apoptosis that was the main mechanism of cell death when AKM were infected with bacteria.

並列關鍵字

apoptosis ； free radicals ； macrophage ； non-specific immunity ； tilapia